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Mouse Anti-DUT Recombinant Antibody (1C9) (CBMAB-D1920-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1C9
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
DUT (AAH33645, 68 a.a. ~ 164 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Deoxyuridine Triphosphatase
Introduction
DUT is an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Entrez Gene ID
1854
UniProt ID
P33316
Alternative Names
Deoxyuridine Triphosphatase; DUTP Pyrophosphatase; DUTP Diphosphatase; EC 3.6.1.23; DUTPase; Deoxyuridine 5-Triphosphate Nucleotidohydrolase, Mitochondrial; DUTP Nucleotidohydrolase;
Research Area
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Biological Process
DNA replication Source: ProtInc
dUMP biosynthetic process Source: GO_Central
dUTP catabolic process Source: GO_Central
Nucleobase-containing compound metabolic process Source: ProtInc
Cellular Location
Isoform 2: Nucleus
Isoform 3: Mitochondrion
PTM
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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