Mouse Anti-DUT Recombinant Antibody (D1920) (V2LY-0425-LY1100)

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Basic Information

Host Animal
Mouse
Clone
D1920
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 203-219 near the C-terminus of human dUTPase.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Pig
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Deoxyuridine Triphosphatase
Entrez Gene ID
UniProt ID
Research Area
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Biological Process
DNA replication Source: ProtInc
dUMP biosynthetic process Source: GO_Central
dUTP catabolic process Source: GO_Central
Nucleobase-containing compound metabolic process Source: ProtInc
Cellular Location
Isoform 2: Nucleus
Isoform 3: Mitochondrion
PTM
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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