Mouse Anti-DUT Recombinant Antibody (D1920) (V2LY-0425-LY1100)

Name: Mouse Anti-DUT Recombinant Antibody (D1920)
SKU: V2LY-0425-LY1100
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle, Pig

Clone

D1920

Antibody Isotype

IgG3, κ

Application

WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen

Amino acids 203-219 near the C-terminus of human dUTPase.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle, Pig

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Deoxyuridine Triphosphatase

Entrez Gene ID

1854

UniProt ID

P33316

Research Area

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Biological Process

DNA replication Source: ProtInc
dUMP biosynthetic process Source: GO_Central
dUTP catabolic process Source: GO_Central
Nucleobase-containing compound metabolic process Source: ProtInc

Cellular Location

Isoform 2: Nucleus
Isoform 3: Mitochondrion

PTM

Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.

More Infomation

References

Weng, G. X., Ling, T., Hou, W., Li, S. N., Chen, T., Zhang, Z., ... & Xu, L. G. (2021). Mitochondrial DUT-M potentiates RLR-mediated antiviral signaling by enhancing VISA and TRAF2 association. Molecular Immunology, 132, 117-125.

Pérez-Moreno, G., Sánchez-Carrasco, P., Ruiz-Pérez, L. M., Johansson, N. G., Müller, S., Baragaña, B., ... & González-Pacanowska, D. (2019). Validation of Plasmodium falciparum dUTPase as the target of 5′-tritylated deoxyuridine analogues with anti-malarial activity. Malaria journal, 18(1), 1-12.

Ye, F., Wang, H., Liu, J., Cheng, Q., Chen, X., & Chen, H. (2019). Genetic variants of the dUTPase-encoding gene DUT increase HR-HPV infection rate and cervical squamous cell carcinoma risk. Scientific reports, 9(1), 1-9.

Alam, M. S., Moriyama, H., & Matsumoto, M. (2018). Inhibition of Dr‐dut gene causes DNA damage in planarian. Molecular reproduction and development, 85(3), 188-196.

Ji, D., Kietrys, A. M., Lee, Y., & Kool, E. T. (2018). ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase. Bioconjugate chemistry, 29(5), 1614-1621.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

DUT Matched Antibody Pair (355)

Related Products

Mouse Anti-DUT Recombinant Antibody (CBYCD-451)

Mouse Anti-DUT Recombinant Antibody (1C9)

DUT Matched Antibody Pair (355)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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