Mouse Anti-EPOR (Phospho-Tyr426) Recombinant Antibody (CBFYE-1071) (V2LY-0825-LY548)

Name: Mouse Anti-EPOR (Phospho-Tyr426) Recombinant Antibody (CBFYE-1071)
SKU: V2LY-0825-LY548
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1071

Application

Immunogen

Phosphopeptide containing the human EPOR Y426 site.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Erythropoietin Receptor

Entrez Gene ID

2057

UniProt ID

P19235

Research Area

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

Biological Process

Brain development Source: Ensembl
Decidualization Source: Ensembl
Heart development Source: Ensembl
Signal transduction Source: ProtInc

Cellular Location

Cell membrane
Isoform EPOR-S: Secreted. Secreted and located to the cell surface.

Involvement in disease

Erythrocytosis, familial, 1 (ECYT1):
An autosomal dominant disorder characterized by elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.

Topology

Extracellular: 25-250
Helical: 251-273
Cytoplasmic: 274-508

PTM

On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

Mochida, Y., Hara, A., Oka, M., Maesato, K., Ishioka, K., Moriya, H., ... & Wada, T. (2022). Association between Anti-Erythropoietin Receptor Antibodies and Cardiac Function in Patients on Hemodialysis: A Multicenter Cross-Sectional Study. Biomedicines, 10(9), 2092.

Papic, M., Zivanovic, S., Vucicevic, T., Papic, M. V., Zdravkovic, D., Milivojevic, N., ... & Popovic, M. (2022). Pulpal expression of erythropoietin and erythropoietin receptor after direct pulp capping in rat. European Journal of Oral Sciences, e12888.

Ramshekar, A., Bretz, C. A., Kunz, E., Cung, T., Richards, B. T., Stoddard, G. J., ... & Hartnett, M. E. (2022). Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization. Biomedicines, 10(7), 1655.

Nijholt, K. T., Meems, L. M., Ruifrok, W., Maass, A. H., Yurista, S. R., Pavez-Giani, M. G., ... & Westenbrink, B. D. (2021). The erythropoietin receptor expressed in skeletal muscle is essential for mitochondrial biogenesis and physiological exercise. Pflügers Archiv-European Journal of Physiology, 473(8), 1301-1313.

Oshima, M., Hara, A., Toyama, T., Jun, M., Pollock, C., Jardine, M., ... & Wada, T. (2021). Comparison of circulating biomarkers in predicting diabetic kidney disease progression with autoantibodies to erythropoietin receptor. Kidney international reports, 6(2), 284-295.

Vukelic, J., Dobrila-Dintinjana, R., Marijic, B., Marzic, D., Braut, T., & Velepic, M. (2021). New insights into erythropoietin and erythropoietin receptor in laryngeal cancer tissue. Medicine, 100(3).

Wu, B. W., Liu, Y., Wu, M. S., Meng, Y. H., Lu, M., Guo, J. D., & Zhou, Y. H. (2020). Downregulation of microRNA‐135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor. IUBMB life, 72(2), 198-213.

Bretz, C. A., Simmons, A. B., Kunz, E., Ramshekar, A., Kennedy, C., Cardenas, I., & Hartnett, M. E. (2020). Erythropoietin receptor signaling supports retinal function after vascular injury. The American journal of pathology, 190(3), 630-641.

Shi, M., Flores, B., Li, P., Gillings, N., McMillan, K. L., Ye, J., ... & Hu, M. C. (2018). Effects of erythropoietin receptor activity on angiogenesis, tubular injury, and fibrosis in acute kidney injury: a “U-shaped” relationship. American Journal of Physiology-Renal Physiology, 314(4), F501-F516.

Ostrowski, D., & Heinrich, R. (2018). Alternative erythropoietin receptors in the nervous system. Journal of Clinical Medicine, 7(2), 24.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

EPOR Matched Antibody Pair (387) (CAT#: APMAB-387LY)

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Isotype control

For research use only. Not intended for any clinical use.

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