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Mouse Anti-EPOR (AA 31-130) Recombinant Antibody (CBFYE-1073) (CBMAB-E1600-FY)

This product is mouse antibody that recognizes EPOR. The antibody CBFYE-1073 can be used for immunoassay techniques such as: ELISA, IP, WB.
See all EPOR antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-1073
Antibody Isotype
IgG2b, κ
Application
ELISA, IP, WB

Basic Information

Immunogen
EPOR partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: PDPKFESKAA LLAARGPEEL LCFTERLEDL VCFWEEAASA GVGPGNYSFS YQLEDEPWKL CRLHQAPTAR GAVRFWCSLP TADTSSFVPL ELRVTAASGA
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 31-130

Target

Full Name
Erythropoietin Receptor
Introduction
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
Entrez Gene ID
2057
UniProt ID
P19235
Alternative Names
Erythropoietin Receptor; EPO-R
Research Area
Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
Biological Process
Brain development Source: Ensembl
Decidualization Source: Ensembl
Heart development Source: Ensembl
Signal transduction Source: ProtInc
Cellular Location
Cell membrane
Isoform EPOR-S: Secreted. Secreted and located to the cell surface.
Involvement in disease
Erythrocytosis, familial, 1 (ECYT1):
An autosomal dominant disorder characterized by elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
Topology
Extracellular: 25-250
Helical: 251-273
Cytoplasmic: 274-508
PTM
On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

Mochida, Y., Hara, A., Oka, M., Maesato, K., Ishioka, K., Moriya, H., ... & Wada, T. (2022). Association between Anti-Erythropoietin Receptor Antibodies and Cardiac Function in Patients on Hemodialysis: A Multicenter Cross-Sectional Study. Biomedicines, 10(9), 2092.

Papic, M., Zivanovic, S., Vucicevic, T., Papic, M. V., Zdravkovic, D., Milivojevic, N., ... & Popovic, M. (2022). Pulpal expression of erythropoietin and erythropoietin receptor after direct pulp capping in rat. European Journal of Oral Sciences, e12888.

Ramshekar, A., Bretz, C. A., Kunz, E., Cung, T., Richards, B. T., Stoddard, G. J., ... & Hartnett, M. E. (2022). Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization. Biomedicines, 10(7), 1655.

Nijholt, K. T., Meems, L. M., Ruifrok, W., Maass, A. H., Yurista, S. R., Pavez-Giani, M. G., ... & Westenbrink, B. D. (2021). The erythropoietin receptor expressed in skeletal muscle is essential for mitochondrial biogenesis and physiological exercise. Pflügers Archiv-European Journal of Physiology, 473(8), 1301-1313.

Oshima, M., Hara, A., Toyama, T., Jun, M., Pollock, C., Jardine, M., ... & Wada, T. (2021). Comparison of circulating biomarkers in predicting diabetic kidney disease progression with autoantibodies to erythropoietin receptor. Kidney international reports, 6(2), 284-295.

Vukelic, J., Dobrila-Dintinjana, R., Marijic, B., Marzic, D., Braut, T., & Velepic, M. (2021). New insights into erythropoietin and erythropoietin receptor in laryngeal cancer tissue. Medicine, 100(3).

Wu, B. W., Liu, Y., Wu, M. S., Meng, Y. H., Lu, M., Guo, J. D., & Zhou, Y. H. (2020). Downregulation of microRNA‐135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor. IUBMB life, 72(2), 198-213.

Bretz, C. A., Simmons, A. B., Kunz, E., Ramshekar, A., Kennedy, C., Cardenas, I., & Hartnett, M. E. (2020). Erythropoietin receptor signaling supports retinal function after vascular injury. The American journal of pathology, 190(3), 630-641.

Shi, M., Flores, B., Li, P., Gillings, N., McMillan, K. L., Ye, J., ... & Hu, M. C. (2018). Effects of erythropoietin receptor activity on angiogenesis, tubular injury, and fibrosis in acute kidney injury: a “U-shaped” relationship. American Journal of Physiology-Renal Physiology, 314(4), F501-F516.

Ostrowski, D., & Heinrich, R. (2018). Alternative erythropoietin receptors in the nervous system. Journal of Clinical Medicine, 7(2), 24.

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For research use only. Not intended for any clinical use.

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