BATF3 Antibodies

Background

BATF3, as a transcription factor belonging to the basic leucine zipper family, plays a crucial role in the development and differentiation of lymphocytes. This protein binds to specific DNA sequences through homodimerization or heterodimerization, precisely regulating the expression of target genes, especially participating in the lineage specialization and functional maintenance of CD8α+ dendritic cells. The immune pathway mediated by BATF3 is essential for initiating antiviral responses and anti-tumor immune surveillance in the body. Abnormal function of BATF3 will directly weaken the activation efficiency of cytotoxic T cells. Initially identified as a member of the AP-1 family in the early 2000s, BATF3 was later confirmed to be an indispensable regulatory node in the development of classical type I dendritic cells. The breakthrough work has provided new targets for tumor immunotherapy and vaccine development. The simple and precise regulatory network of this molecule is continuously revealing the deep logic of the immune system in maintaining homeostasis and pathological states.

Structure Function Application Advantage Our Products

Structure of BATF3

BATF3 is an alkaline leucine zipper transcription factor with a molecular weight of approximately 16.8 kDa. There is a slight variation in its sequence among different species.

Species	Human	Mouse	Rat	Bovine
Molecular Weight (kDa)	16.8	17.1	17.0	16.9
Primary Structural Differences	Leucine zipper and DNA-binding domain are highly conserved	About 85% homology with human	Similar to mice	Adaptive variation

BATF3 contains approximately 142 amino acids. Its protein structure is centered around an acidic domain and a leucine zipper. The former is responsible for recognizing AP-1-like sites on DNA, while the latter mediates the formation of a dimer with Jun family proteins. This dimer regulates the transcription of target genes by inserting into specific DNA sequences. BATF3 does not contain an endogenous activation domain and typically functions by interacting with other transcription factors. It plays a decisive role in the differentiation of CD8α+ dendritic cells and in antiviral immunity.

Fig. 1 Members of the BATF family possess both a bZIP and a DBD.¹

Key structural properties of BATF3:

The basic domain is responsible for DNA recognition and binding
The leucine zipper mediates dimerization with Jun family proteins
Lack of classical transcriptional activation domain structure, rely on each other for protein function

Functions of BATF3

The main function of BATF3 is to regulate the differentiation of immune cells and the anti-infection response. Additionally, it also plays a role in various physiological processes, including metabolic adaptation and tumor immune surveillance.

Function	Description
Dendritic Cell Differentiation	BATF3 is a crucial transcription factor necessary for the development of CD8α+ classical dendritic cells.
Antiviral Immunity	By regulating the expression of target genes, it participates in initiating the cytotoxic T cell response against intracellular pathogens.
Tumor immune surveillance	Maintaining the antigen cross-presentation ability of dendritic cells is conducive to the recognition and elimination of tumor cells by effector T cells.
Metabolic Adaptation	Regulates mitochondrial function and energy metabolism in specific immune subsets, influencing cell survival and effector functions.
T Cell Function Support	Although it does not directly activate transcription, it cooperatively regulates T cell differentiation and cytokine expression through dimerization with Jun protein.

BATF3 itself lacks the typical trans-activating domain. Its function is highly dependent on the recruitment of dimerization partners and auxiliary factors, and it acts as a hub node rather than a terminal executor in the immune transcriptional regulatory network.

Applications of BATF3 and BATF3 Antibody in Literature

1. Wang, Xiaomeng, et al. "The role of BATF in immune cell differentiation and autoimmune diseases." Biomarker Research 13.1 (2025): 22. https://doi.org/10.1186/s40364-025-00733-x

The article indicates that BATF, as a member of the AP-1 family, regulates the differentiation and function of immune cells, and participates in the progression of autoimmune diseases, allergies, and tumors, providing new directions for related treatment strategies.

2. Hamade, Hussein, et al. "BATF3 protects against metabolic syndrome and maintains intestinal epithelial homeostasis." Frontiers in Immunology 13 (2022): 841065. https://doi.org/10.3389/fimmu.2022.841065

The article indicates that the absence of BATF3 disrupts the intestinal barrier and leads to dysbiosis (such as a decrease in Akkermansia), thereby triggering metabolic syndrome; inhibiting glycolysis or using antibiotics can alleviate these symptoms, suggesting that it plays a protective role in maintaining intestinal homeostasis.

3. Reschke, Robin, and Daniel J. Olson. "Leveraging STING, batf3 dendritic cells, CXCR3 ligands, and other components related to innate immunity to induce A "Hot" Tumor microenvironment that is responsive to immunotherapy." Cancers 14.10 (2022): 2458. https://doi.org/10.3390/cancers14102458

The article indicates that the tumor immune response relies on Batf3-expressing CD103+ DCs for the activation and recruitment of T cells. For the absence of this mechanism in "cold tumors", strategies such as delivering STING agonists or activating DCs can enhance the efficacy of immunotherapy.

4. Benckendorff, Julian, et al. "Usefulness of BATF3 immunohistochemistry in diagnosing classical Hodgkin lymphoma." Diagnostics 11.6 (2021): 1123. https://doi.org/10.3390/diagnostics11061123

The article indicates that BATF3 is stably and highly expressed in classical Hodgkin lymphoma and anaplastic large cell lymphoma, and can serve as a sensitive and specific immunohistochemical diagnostic marker, which is helpful for differentiating it from other lymphoma subtypes.

5. Lee, Woo Ho, et al. "BATF3 is sufficient for the induction of Il9 expression and can compensate for BATF during Th9 cell differentiation." Experimental & molecular medicine 51.11 (2019): 1-12. https://doi.org/10.1038/s12276-019-0348-6

The article indicates that in the differentiation of Th9 cells, the OX40 signal induces the expression of BATF3. BATF3, by binding to IRF4, activates IL-9, which can replace the function of BATF and thereby drive the inflammatory responses associated with allergies and asthma.

Creative Biolabs: BATF3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality BATF3 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

Custom BATF3 Antibody Development: Tailor-made solutions to meet specific research requirements.
Bulk Production: Large-scale antibody manufacturing for industry partners.
Technical Support: Expert consultation for protocol optimization and troubleshooting.
Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our BATF3 antibodies, custom preparations, or technical support, contact us at info@creative-biolabs.com.

Reference

Wang, Xiaomeng, et al. "The role of BATF in immune cell differentiation and autoimmune diseases." Biomarker Research 13.1 (2025): 22. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1186/s40364-025-00733-x