Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY)

Name: Mouse Anti-CHRNA9 Recombinant Antibody (8E4)
SKU: CBMAB-C9161-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY) in WB

Quantitative analysis of α9-containing nAChRs by Western blot in various cancer cell lines and breast epithelial cell lines. GAPDH was the control in all experiments. The proteins were detected with the corresponding antibody and an anti-mouse IgG secondary antibody conjugated to horseradish peroxidase (HRP). Each bar is the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, breast cancer cell lines vs. breast epithelial cell lines HS578BST (normal cell control). ...View More

Sun, Z., Zhangsun, M., Dong, S., Liu, Y., Qian, J., Zhangsun, D., & Luo, S. (2020). Differential expression of nicotine acetylcholine receptors associates with human breast cancer and mediates antitumor activity of αO-conotoxin gexiva. Marine drugs, 18(1), 61.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY) in WB

Western blot images of α3, α4, α5, α6, α7, α9, α10, β2, β3, and β4 nAChR. ...View More

Liu, Y., Qian, J., Sun, Z., Zhangsun, D., & Luo, S. (2019). Cervical cancer correlates with the differential expression of nicotinic acetylcholine receptors and reveals therapeutic targets. Marine drugs, 17(5), 256.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8E4

Application

ELISA, WB, IP

Immunogen

Amino acids 139-221 of AChRα9 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cholinergic receptor, nicotinic, alpha 9

Introduction

This gene is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. [provided by RefSeq, Feb 2012]

Entrez Gene ID

55584

UniProt ID

Q9UGM1

Alternative Names

Cholinergic Receptor Nicotinic Alpha 9 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 9; Cholinergic Receptor, Nicotinic, Alpha 9 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 9; NACHRA9; Acetylcholine Receptor, Neuronal Nicotinic, Alpha-

Function

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane (PubMed:11752216, PubMed:25282151).
The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane (PubMed:11752216, PubMed:25282151).
In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion (PubMed:11021840).

Biological Process

Chemical synaptic transmission Source: GO_Central
Detection of mechanical stimulus involved in sensory perception of sound Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Ion transmembrane transport Source: GO_Central
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Nervous system process Source: GO_Central
Positive regulation of cytosolic calcium ion concentration Source: MGI
Regulation of membrane potential Source: GO_Central
Signal transduction Source: GO_Central

Cellular Location

Postsynaptic cell membrane; Cell membrane

Topology

Extracellular: 26-237
Helical: 238-262
Helical: 269-287
Helical: 302-323
Cytoplasmic: 324-457
Helical: 458-476

PTM

N-glycosylated.

Briggs, F. B. (2021). Nicotinic acetylcholine receptors α7 and α9 modifies tobacco smoke risk for multiple sclerosis. Multiple Sclerosis Journal, 27(8), 1166-1174.

Morley, B. J., Whiteaker, P., & Elgoyhen, A. B. (2018). Commentary: Nicotinic acetylcholine receptor α9 and α10 subunits are expressed in the brain of mice. Frontiers in cellular neuroscience, 12, 104.

Zakrzewicz, A., Richter, K., Agné, A., Wilker, S., Siebers, K., Fink, B., ... & Grau, V. (2017). Canonical and novel non-canonical cholinergic agonists inhibit ATP-induced release of monocytic interleukin-1β via different combinations of nicotinic acetylcholine receptor subunits α7, α9 and α10. Frontiers in cellular neuroscience, 11, 189.

Huang, L. C., Lin, C. L., Qiu, J. Z., Lin, C. Y., Hsu, K. W., Tam, K. W., ... & Lee, C. H. (2017). Nicotinic acetylcholine receptor subtype alpha-9 mediates triple-negative breast cancers based on a spontaneous pulmonary metastasis mouse model. Frontiers in cellular neuroscience, 11, 336.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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