MCM8

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Full Name

minichromosome maintenance complex component 8

Function

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855).

Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093).

Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855).

The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855).

However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G1 phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404).

Probably by regulating HR, plays a key role during gametogenesis (By similarity).

Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093).

Biological Process

Cell cycle Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: UniProtKB
Double-strand break repair via homologous recombination Source: UniProtKB
Female gamete generation Source: UniProtKB
Male gamete generation Source: UniProtKB
Protein localization to chromatin Source: UniProtKB
Protein stabilization Source: UniProtKB
Recombinational interstrand cross-link repair Source: UniProtKB

Cellular Location

Nucleus
Other locations
Chromosome
Note: Localizes to nuclear foci (PubMed:26215093). Localizes to double-stranded DNA breaks (PubMed:23401855). Binds chromatin throughout the cell cycle (PubMed:15684404).

Involvement in disease

Premature ovarian failure 10 (POF10):
An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.