Mouse Anti-MCM8 Recombinant Antibody (CBFYM-0610) (CBMAB-M0743-FY)

Name: Mouse Anti-MCM8 Recombinant Antibody (CBFYM-0610)
SKU: CBMAB-M0743-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0610

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

minichromosome maintenance complex component 8

Introduction

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Entrez Gene ID

84515

UniProt ID

Q9UJA3

Alternative Names

Minichromosome Maintenance 8 Homologous Recombination Repair Factor; Minichromosome Maintenance Complex Component 8; C20orf154; MCM8 Minichromosome Maintenance Deficient 8; DNA Replication Licensing Factor MCM8; Chromosome 20 Open Reading Frame 154; Minichromosome Maintenance 8

Function

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855).

Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093).

Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855).

The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855).

However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G1 phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404).

Probably by regulating HR, plays a key role during gametogenesis (By similarity).

Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093).

Biological Process

Cell cycle Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: UniProtKB
Double-strand break repair via homologous recombination Source: UniProtKB
Female gamete generation Source: UniProtKB
Male gamete generation Source: UniProtKB
Protein localization to chromatin Source: UniProtKB
Protein stabilization Source: UniProtKB
Recombinational interstrand cross-link repair Source: UniProtKB

Cellular Location

Nucleus
Other locations
Chromosome
Note: Localizes to nuclear foci (PubMed:26215093). Localizes to double-stranded DNA breaks (PubMed:23401855). Binds chromatin throughout the cell cycle (PubMed:15684404).

Involvement in disease

Premature ovarian failure 10 (POF10):
An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

Griffin, W. C., McKinzey, D. R., Klinzing, K. N., Baratam, R., Eliyapura, A., & Trakselis, M. A. (2022). A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress. Nature Communications, 13(1), 5090.

Dondik, Y., Lei, Z., Gaskins, J., & Pagidas, K. (2019). Minichromosome maintenance complex component 8 and 9 gene expression in the menstrual cycle and unexplained primary ovarian insufficiency. Journal of Assisted Reproduction and Genetics, 36, 57-64.

Griffin, W. C., & Trakselis, M. A. (2019). The MCM8/9 complex: A recent recruit to the roster of helicases involved in genome maintenance. DNA repair, 76, 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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