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Mouse Anti-ABL2 Recombinant Antibody (V2-179121) (CBMAB-A0364-YC)

Provided herein is a Mouse monoclonal antibody against Human ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase. The antibody can be used for immunoassay techniques, such as ELISA, PLA, RNAi, WB.
See all ABL2 antibodies
Published Data
Mouse Anti-ABL2 Recombinant Antibody (6D5) (CBMAB-A0364-YC) in WB
Immunoblots of ABL2 in HCC1954-LCC1 cells virally transduced with Cas9 and gRNAs targeting ABL2 (ABL2 knockout [KO] ). Control cells are virally transduced with empty gRNA backbones (control). ...View More
McKernan, C. M., Khatri, A., Hannigan, M., Child, J., Chen, Q., Mayro, B., ... & Pendergast, A. M. (2022). ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain. Cell reports, 40(9), 111268.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179121
Antibody Isotype
IgG2a, κ
Application
ELISA, PLA, RNAi, WB

Basic Information

Immunogen
ABL2 (AAH65912, 743-842 aa) partial recombinant protein with GST tag. Immunogen sequence: KKTLGLRAGK PTASDDTSKP FPRSNSTSSM SSGLPEQDRM AMTLPRNCQR SKLQLERTVS TSSQPEENVD RANDMLPKKS EESAAPSRER PKAKLLPRGA
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
In situ Proximity Ligation Assay1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 743-842

Target

Full Name
ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase
Introduction
ABL2 is a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through i
Entrez Gene ID
27
UniProt ID
P42684
Alternative Names
ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase; V-Abl Abelson Murine Leukemia Viral Oncogene Homolog 2; C-Abl Oncogene 2, Non-Receptor Tyrosine Kinase; Abelson-Related Gene Protein; Tyrosine-Protein Kinase ARG; EC 2.7.10.2; ABLL;
Function
Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Biological Process
Cell adhesion
Cellular protein modification process
Cellular response to retinoic acid
Cytokine-mediated signaling pathway
Exploration behavior
Negative regulation of Rho protein signal transduction
Peptidyl-tyrosine phosphorylation
Positive regulation of cytosolic calcium ion concentration
Positive regulation of neuron projection development
Positive regulation of oxidoreductase activity
Positive regulation of phospholipase C activity
Regulation of actin cytoskeleton reorganization
Regulation of autophagy
Regulation of cell adhesion
Regulation of cell motility
Regulation of endocytosis
Signal transduction
Cellular Location
Cytoskeleton
PTM
Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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