Mouse Anti-ABL2 Recombinant Antibody (V2-179121) (CBMAB-A0364-YC)

Name: Mouse Anti-ABL2 Recombinant Antibody (V2-179121)
SKU: CBMAB-A0364-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ABL2 Recombinant Antibody (6D5) (CBMAB-A0364-YC) in WB

Immunoblots of ABL2 in HCC1954-LCC1 cells virally transduced with Cas9 and gRNAs targeting ABL2 (ABL2 knockout [KO] ). Control cells are virally transduced with empty gRNA backbones (control). ...View More

McKernan, C. M., Khatri, A., Hannigan, M., Child, J., Chen, Q., Mayro, B., ... & Pendergast, A. M. (2022). ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain. Cell reports, 40(9), 111268.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179121

Application

ELISA, PLA, RNAi, WB

Immunogen

ABL2 (AAH65912, 743-842 aa) partial recombinant protein with GST tag. Immunogen sequence: KKTLGLRAGK PTASDDTSKP FPRSNSTSSM SSGLPEQDRM AMTLPRNCQR SKLQLERTVS TSSQPEENVD RANDMLPKKS EESAAPSRER PKAKLLPRGA

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
In situ Proximity Ligation Assay	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 743-842

More Infomation

Target

Full Name

ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase

Introduction

ABL2 is a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through i

Entrez Gene ID

UniProt ID

P42684

Alternative Names

ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase; V-Abl Abelson Murine Leukemia Viral Oncogene Homolog 2; C-Abl Oncogene 2, Non-Receptor Tyrosine Kinase; Abelson-Related Gene Protein; Tyrosine-Protein Kinase ARG; EC 2.7.10.2; ABLL;

Function

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Biological Process

Cell adhesion
Cellular protein modification process
Cellular response to retinoic acid
Cytokine-mediated signaling pathway
Exploration behavior
Negative regulation of Rho protein signal transduction
Peptidyl-tyrosine phosphorylation
Positive regulation of cytosolic calcium ion concentration
Positive regulation of neuron projection development
Positive regulation of oxidoreductase activity
Positive regulation of phospholipase C activity
Regulation of actin cytoskeleton reorganization
Regulation of autophagy
Regulation of cell adhesion
Regulation of cell motility
Regulation of endocytosis
Signal transduction

Cellular Location

Cytoskeleton

PTM

Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.

Creeden, J. F., Alganem, K., Imami, A. S., Brunicardi, F. C., Liu, S. H., Shukla, R., ... & McCullumsmith, R. E. (2020). Kinome Array Profiling of Patient-Derived Pancreatic Ductal Adenocarcinoma Identifies Differentially Active Protein Tyrosine Kinases. International journal of molecular sciences, 21(22), 8679.

Jacobsen, F. A., Scherer, A. N., Mouritsen, J., Bragadóttir, H., Bäckström, B. T., Sardar, S., ... & Andersson, Å. (2018). A role for the non-receptor tyrosine kinase Abl2/Arg in experimental neuroinflammation. Journal of Neuroimmune Pharmacology, 13(2), 265-276.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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