What applications has this antibody been validated for?

The Rat Anti-ADGRE4 Recombinant Antibody (V2-160163) (CBMAB-F0011-CQ) has been validated for use in Western Blot, Immunohistochemistry, and Flow Cytometry.

Is this antibody suitable for detecting native ADGRE4 protein?

Yes, this recombinant antibody is designed to detect native Rat ADGRE4 protein in appropriate sample types and applications where the epitope is accessible.

Rat Anti-ADGRE4 Recombinant Antibody (V2-160163) (CBMAB-F0011-CQ)

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Published Data

Rat Anti-ADGRE4 Recombinant Antibody (6F12) (CBMAB-F0011-CQ) in FC

Immunofluorecence of FIRE staining on various cell types. CHO cells expressing FIRE-FLAG (CHO-FIRE) were stained with anti-FIRE mAb 3H7 or 6F12. All other cell types were stained with anti-FIRE mAb 6F12. The anti-FIRE mAb were biotinylated and then detected in flow cytometry using streptavidin-PE second stage. Other markers were used to gate the cell type of interest during flow cytometry. Anti-CD3-FITC was used to identify T cells, anti-CD19-FITC, and anti-B220-Cy5 double markers to identify splenic B cells. Splenic macrophages were identified by double staining with anti-CD11b-Cy-5 and anti-F4/80-FITC. Peritoneal macrophages were identified by bright staining with anti-CD11b-Cy5. DC were identified by staining with anti-CD11c-FITC and high forward light scatter. Splenic DC were further stained with anti-CD4-Alexa and anti-CD8-Cy5 and were then gated on the populations that expressed CD4, CD8, or neither of these markers. The continuous line represents the anti-FIRE staining on gated cells. The background control staining is indicated by the dotted line, representing staining of control CHO-Neo in the case of CHO-FIRE cells and of an isotype matched control stain in all other cases. Histographs are on a logarithmic scale. ...View More

Caminschi, I., Lucas, K. M., O'Keeffe, M. A., Hochrein, H., Laâbi, Y., Köntgen, F., ... & Wright, M. D. (2001). Molecular cloning of F4/80-like-receptor, a seven-span membrane protein expressed differentially by dendritic cell and monocyte-macrophage subpopulations. The Journal of Immunology, 167(7), 3570-3576.

Rat Anti-ADGRE4 Recombinant Antibody (6F12) (CBMAB-F0011-CQ) in IHC

Immunohistological analysis of FIRE expression. Frozen tissue sections of spleens were stained in red with an isotype control mAb (A and D) or anti-FIRE mAb 6F12 (B, C, E, and F). Sections were stained in blue with anti-CD11c mAb (C), MOMA-1 (D and E), and anti-MadCAM-1 (F). FIRE cells reside predominantly in the red pulp (RP) and in the marginal zone (MZ) of the spleen, but very few FIRE cells are seen in the white pulp (WP). Frozen sections of lymph nodes were stained in red with the anti-FIRE mAb. Some FIRE cells are seen in the subcapsular sinus (H) and less frequently in parts of the paracortex (I). Staining authenticity is confirmed with an isotype control Ab that fails to stain lymph nodes (G). Lymph node sections were counterstained with hematoxylin. ...View More

Datasheet

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Basic Information

Host Animal

Rat

Clone

V2-160163

Application

IHC-F, FC, IP, WB

Immunogen

CHO cells expressing recombinant FIRE fusion protein

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

ABS

Preservative

< 0.09% sodium azide

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

adhesion G protein-coupled receptor E4

Entrez Gene ID

52614

UniProt ID

Q91ZE5

Alternative Names

Adgre4; Egf-tm7; Fire; Gpr127; D17Ertd479e; Emr4; Adgre4p; Emr4p

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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