Mouse Anti-CASQ1 Recombinant Antibody (CBFYC-0863) (CBMAB-C0918-FY)

Name: Mouse Anti-CASQ1 Recombinant Antibody (CBFYC-0863)
SKU: CBMAB-C0918-FY
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Published Data

Mouse Anti-CASQ1 Recombinant Antibody (CBFYC-0863) (CBMAB-C0918-FY) in WB

Expression of the EV marker proteins in plasma and SkM-interstitium EVs. Plasma and interstitium EVs were isolated from two mice using PS-affinity (left) or CD81-affinity (right) beads. ...View More

Watanabe, S., Sudo, Y., Makino, T., Kimura, S., Tomita, K., Noguchi, M., ... & Yamauchi, Y. (2022). Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment. PNAS nexus, 1(4), pgac173.

Mouse Anti-CASQ1 Recombinant Antibody (CBFYC-0863) (CBMAB-C0918-FY) in IP

Co-immunoprecipitation between CSQ1 and STIM1. Whole cell lysates were immunoprecipitated with specific anti-CSQ1 mAb and protein-G agarose, then Western blot was performed with specific anti-STIM1mAb (left). STIM1-CSQ1 association intensities were quantified as average protein ratios ± SD of STIM1/CSQ1 (right). ...View More

Wang, L., Zhang, L., Li, S., Zheng, Y., Yan, X., Chen, M., ... & Luo, D. (2015). Retrograde regulation of STIM1-Orai1 interaction and store-operated Ca2+ entry by calsequestrin. Scientific reports, 5(1), 11349.

Mouse Anti-CASQ1 Recombinant Antibody (CBFYC-0863) (CBMAB-C0918-FY) in IF

STIM1 aggregation and redistribution due to Ca2+ store depletion in HEK293 cells transfected with different plasmids. Scale bar = 10 μm. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0863

Application

ELISA, IHC-P, IF, IP, WB

Immunogen

Amino acids 31-90 mapping near the N-terminus of calsequestrin 1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 29-130

More Infomation

Target

Full Name

calsequestrin 1 (fast-twitch, skeletal muscle)

Introduction

CASQ1 (Calsequestrin 1) is a Protein Coding gene. Diseases associated with CASQ1 include Myopathy, Vacuolar, With Casq1 Aggregates and Myopathy, Tubular Aggregate, 1. Among its related pathways are Antiarrhythmic Pathway, Pharmacodynamics and Myometrial Relaxation and Contraction Pathways. Gene Ontology (GO) annotations related to this gene include calcium ion binding. An important paralog of this gene is CASQ2.

Entrez Gene ID

844

UniProt ID

P31415

Alternative Names

Calsequestrin 1; Calmitine; Calsequestrin 1 (Fast-Twitch, Skeletal Muscle); Calsequestrin, Skeletal Muscle Isoform; CASQ; Calsequestrin 1, Fast-Twitch, Skeletal Muscle

Function

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:28895244).
Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca2+ ions (PubMed:28895244).
Regulates the release of lumenal Ca2+ via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca2+ entry (SOCE) activity (PubMed:27185316).

Biological Process

Endoplasmic reticulum organization Source: Ensembl
Ion transmembrane transport Source: Reactome
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Positive regulation of store-operated calcium channel activity Source: UniProtKB
Protein polymerization Source: UniProtKB
Regulation of cardiac conduction Source: Reactome
Regulation of release of sequestered calcium ion into cytosol Source: GO_Central
Regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: UniProtKB
Regulation of store-operated calcium entry Source: UniProtKB
Response to denervation involved in regulation of muscle adaptation Source: Ensembl
Response to heat Source: Ensembl
Response to organic substance Source: Ensembl
Sarcomere organization Source: UniProtKB
Skeletal muscle tissue development Source: Ensembl

Cellular Location

Endoplasmic reticulum; Mitochondrion matrix; Sarcoplasmic reticulum; Sarcoplasmic reticulum lumen; Sarcoplasmic reticulum membrane. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Preferentially forms linear and round aggregates in the endoplasmic reticulum (ER) of resting cells (PubMed:28895244). In a minority of cells, homogeneously detected in the ER lumen (PubMed:28895244). Colocalizes with STIM1 at endoplasmic reticulum in response to a depletion of intracellular calcium (PubMed:27185316).

Involvement in disease

Myopathy, vacuolar, with CASQ1 aggregates (VMCQA): An autosomal dominant mild muscle disorder characterized by adult onset of muscle cramping and weakness as well as increased levels of serum creatine kinase. The disorder is not progressive, and some patients may be asymptomatic.
Myopathy, tubular aggregate, 1 (TAM1): A rare congenital myopathy characterized by regular arrays of membrane tubules on muscle biopsies without additional histopathological hallmarks. Tubular aggregates in muscle are structures of variable appearance consisting of an outer tubule containing either one or more microtubule-like structures or amorphous material. They may occur in a variety of circumstances, including inherited myopathies, alcohol- and drug-induced myopathies, exercise-induced cramps or muscle weakness.

PTM

N-glycosylated.

Sun, Z., Wang, L., Han, L., Wang, Y., Zhou, Y., Li, Q., ... & Luo, D. (2021). Functional Calsequestrin-1 Is Expressed in the Heart and Its Deficiency Is Causally Related to Malignant Hyperthermia-Like Arrhythmia. Circulation, 144(10), 788-804.

Jeong, S. Y., Oh, M. R., Choi, J. H., Woo, J. S., & Lee, E. H. (2021). Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells, 10(11), 2821.

Rossi, D., Gamberucci, A., Pierantozzi, E., Amato, C., Migliore, L., & Sorrentino, V. (2021). Calsequestrin, a key protein in striated muscle health and disease. Journal of Muscle Research and Cell Motility, 42(2), 267-279.

Michelucci, A., Boncompagni, S., Pietrangelo, L., Takano, T., Protasi, F., & Dirksen, R. T. (2020). Pre-assembled Ca2+ entry units and constitutively active Ca2+ entry in skeletal muscle of calsequestrin-1 knockout mice. Journal of General Physiology, 152(10), e202012617.

Guarnier, F. A., Michelucci, A., Serano, M., Pietrangelo, L., Pecorai, C., Boncompagni, S., & Protasi, F. (2018). Aerobic training prevents heatstrokes in calsequestrin-1 knockout mice by reducing oxidative stress. Oxidative medicine and cellular longevity, 2018.

Böhm, J., Lornage, X., Chevessier, F., Birck, C., Zanotti, S., Cudia, P., ... & Laporte, J. (2018). CASQ1 mutations impair calsequestrin polymerization and cause tubular aggregate myopathy. Acta neuropathologica, 135(1), 149-151.

Michelucci, A., Boncompagni, S., Canato, M., Reggiani, C., & Protasi, F. (2017). Estrogens protect calsequestrin-1 knockout mice from lethal hyperthermic episodes by reducing oxidative stress in muscle. Oxidative medicine and cellular longevity, 2017.

Böhm, J., Lornage, X., Zanotti, S., Cudia, P., Schneider-Gold, C., Malfatti, E., ... & Laporte, J. (2017). CASQ1 mutations impair calsequestrin polymerization and cause tubular aggregate myopathy. Neuromuscular Disorders, 27, S176.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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