MPP3 Antibodies

Structure of MPP3

MPP3 is a scaffold protein belonging to the membrane-associated guanylate kinase (MAGUK) family. Its molecular weight is roughly similar among different vertebrate species, approximately ranging from 70 to 75 kDa. The molecular weight difference mainly results from minor variations in its protein domains (such as PDZ, SH3, and GUK domains), which adapt to the different functional requirements for mediating cell polarity and signal transduction in specific cell types (such as inner ear hair cells, epithelial cells).

MPP3 is composed of approximately 550-650 amino acids. Its core feature lies in the tandem PDZ domains, which can specifically recognize and bind to the C-terminal sequences of target proteins (such as cadherins, receptors), thereby assembling multi-protein complexes at specific regions of the cell membrane (such as tight junctions, basal regions of stereocilia). This interaction is crucial for maintaining the integrity of the epithelial barrier and the stability of the mechanical sensing structure of inner ear hair cells. Dysfunction of this function is closely related to diseases such as hearing impairment.

Fig. 1 Schematic representation of the structures of MPP3.¹

Key structural properties of MPP3:

• Has a more conservative structure domain (e.g., PDZ, SH3, GUK), forming a modular framework
• Specifically recognize and bind to the C-terminal sequence of the target protein through the PDZ domain
• As a member of the membrane-associated guanylate kinase (MAGUK) family
• Protein conformation is flexible and tunable to precisely adapt the connectivity and polarity requirements in different cellular environments

Functions of MPP3

The main function of the MPP3 gene is to act as a scaffold protein, participating in the establishment and maintenance of cell polarity, especially in epithelial tissues and sensory hair cells. However, it is also widely involved in various cellular physiological processes, including the assembly of intercellular connections and the regulation of local signaling pathways.

The function of MPP3 relies on its modular domains for specific protein-protein interactions. Its mode of action is highly localized and context-dependent, which is consistent with its core role in precisely integrating structure and signaling in different cell types.

Applications of MPP3 and MPP3 Antibody in Literature

1. Wang, Xinyue, et al. "A novel rabbit anti-myoglobin monoclonal antibody's potential application in rhabdomyolysis associated acute kidney injury."International Journal of Molecular Sciences 24.9 (2023): 7822. https://doi.org/10.1371/journal.pone.0082894

Through the cell spreading experiment in this study, it was first discovered that the allosteric interaction of CADM1 activates the PI3K signaling pathway through the MPP3/Dlg complex, and regulates the cytoskeleton reorganization and epithelial structure formation by relying on Akt and Rac1.

2. Kang, Yoon-A., et al. "Secretory MPP3 reinforce myeloid differentiation trajectory and amplify myeloid cell production." Journal of Experimental Medicine 220.8 (2023): e20230088. https://doi.org/10.1084/jem.20230088

The study found that within the hematopoietic multipotent progenitor cell MPP3, there exists a FcγR-positive subgroup with secretory function, and its endoplasmic reticulum is well-developed. This subgroup not only serves as a reserve pool for granulocyte/macrophage progenitor cells, but also can enhance itself by secreting cytokines during inflammation, rapidly amplifying myeloid generation in the bone marrow microenvironment.

3. Dudok, Jacobus J., et al. "MPP3 is required for maintenance of the apical junctional complex, neuronal migration, and stratification in the developing cortex." Journal of Neuroscience 33.19 (2013): 8518-8527. https://doi.org/10.1523/JNEUROSCI.5627-12.2013

The research has found that during the development of the mammalian cortex, the membrane protein MPP3 is crucial for maintaining the top protein complex and adhesion junctions. The absence of MPP3 leads to disordered spindle orientation, delayed cell migration, and subsequently results in defects in neuronal layering and abnormal localization.

4. Lenaerts, Aurelie, et al. "EBF1 primes B-lymphoid enhancers and limits the myeloid bias in murine multipotent progenitors." Journal of Experimental Medicine 219.11 (2022): e20212437. https://doi.org/10.1084/jem.20212437

The study found that the transcription factor EBF1 regulates the lineage preference of multipotent progenitor cell MPP3 by directly inhibiting C/EBPα, limiting its excessive myeloid differentiation and simultaneously initiating the B lymphocyte fate program.

5. Sakurai-Yageta, Mika, et al. "Dynamic regulation of a cell adhesion protein complex including CADM1 by combinatorial analysis of FRAP with exponential curve-fitting." PLoS One 10.3 (2015): e0116637. https://doi.org/10.1371/journal.pone.0116637

The research found that through long-term FRAP analysis, it was discovered that CADM1 is the core that stabilizes the 4.1B-MPP3 complex. 4.1B and MPP3 exist in two states: free and bound. The ratios are approximately 3:2 and 3:1 respectively, revealing the dynamic regulation mechanism of the cell adhesion complex.

Creative Biolabs: MPP3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality MPP3 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom MPP3 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MPP3 antibodies, custom preparations, or technical support, contact us at email.