Mouse Anti-GSDMA Recombinant Antibody (CBLG1-2983) (CBMAB-G5128-LY)

Name: Mouse Anti-GSDMA Recombinant Antibody (CBLG1-2983)
SKU: CBMAB-G5128-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-2983

Application

ELISA, IP, IF, WB

Immunogen

Amino acids 1-196 mapping at the N-terminus of human gasdermin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

gasdermin A

Entrez Gene ID

Human	284110
Mouse	57911
Rat	360619

UniProt ID

Human	Q96QA5
Mouse	Q9EST1
Rat	D3ZA32

Function

Gasdermin-A:
This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-A, N-terminal) binds to membranes and forms pores, triggering cell death.

Gasdermin-A, N-terminal:
Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:17471240, PubMed:27281216).

Released upon cleavage in vitro of genetically engineered GSDMA, and binds to membrane inner leaflet lipids (PubMed:27281216).

Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (PubMed:27281216).

Binds to membrane inner leaflet lipids, such as phosphatidylinositol (4,5)-bisphosphate (PubMed:27281216).

The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown (PubMed:27281216).

Biological Process

Apoptotic process Source: UniProtKB
Defense response to bacterium Source: GO_Central
Pyroptosis Source: GO_Central

Cellular Location

Gasdermin-A: Cytosol; Perinuclear region
Gasdermin-A, N-terminal: Cell membrane

PTM

Cleavage relieves autoinhibition by releasing the N-terminal moiety (Gasdermin-A, N-terminal) that initiates pyroptosis.

Deng, W., Bai, Y., Deng, F., Pan, Y., Mei, S., Zheng, Z., ... & Liu, X. (2022). Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis. Nature, 602(7897), 496-502.

LaRock, D. L., Johnson, A. F., Wilde, S., Sands, J. S., Monteiro, M. P., & LaRock, C. N. (2022). Group A Streptococcus induces GSDMA-dependent pyroptosis in keratinocytes. Nature, 605(7910), 527-531.

Symmank, J., Jacobs, C., & Schulze-Späte, U. (2022). Suicide signaling by GSDMA: a single-molecule mechanism for recognition and defense against SpeB-expressing GAS. Signal Transduction and Targeted Therapy, 7(1), 153.

Deng, W., Bai, Y., Deng, F., Pan, Y., Mei, S., Zheng, Z., ... & Liu, X. (2022). Author Correction: Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis. Nature, 608(7923), E28-E28.

Gagnon, M. F., Provost, S., Ayachi, S., Buscarlet, M., Mollica, L., Dubé, M. P., & Busque, L. (2021). Quantitative Trait of Neutrophil Count Is Influenced By Variants in the Region of Gsdma and PSMD3-CSF3, aging, Cardiometabolic Comorbidities but Not By Chip. Blood, 138, 1089.

Gatineau-Sailliant, S., Glisovic, S., Gagne, V., Laverdière, C., Leclerc, J. M., Silverman, L. B., ... & Pastore, Y. (2019). Impact of DARC, GSDMA and CXCL2 polymorphisms on induction toxicity in children with acute lymphoblastic leukemia: A complementary study. Leukemia research, 86, 106228.

Madore, A. M., Pain, L., Boucher-Lafleur, A. M., Meloche, J., Morin, A., Simon, M. M., ... & Laprise, C. (2019). GSDMA drives the most replicated association with asthma in naïve CD4+ T cells. bioRxiv, 774760.

Moreno-Moral, A., Bagnati, M., Koturan, S., Ko, J. H., Fonseca, C., Harmston, N., ... & Petretto, E. (2018). Changes in macrophage transcriptome associate with systemic sclerosis and mediate GSDMA contribution to disease risk. Annals of the rheumatic diseases, 77(4), 596-601.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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