Rat Anti-EMCN Recombinant Antibody (28) (CBMAB-E0280-FY)

Name: Rat Anti-EMCN Recombinant Antibody (28)
SKU: CBMAB-E0280-FY
Rating: 5 (1 reviews)

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Published Data

Rat Anti-EMCN Recombinant Antibody (28) (CBMAB-E0280-FY) in IF

a,b, Immunofluorescence of CD31+ (green) or endomucin (EMCN)+ (red) blood vessels, representative image in a and quantification in b. Nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). ...View More

Grockowiak, E., Korn, C., Rak, J., Lysenko, V., Hallou, A., Panvini, F. M., ... & Méndez-Ferrer, S. (2023). Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms. Nature Cancer, 4(8), 1193-1209.

Rat Anti-EMCN Recombinant Antibody (28) (CBMAB-E0280-FY) in IF

Representative images of immunofluorescent analysis of CD31, an angiogenesis marker (green), Emcn, an endothelial cell marker (red) and nuclei (4',6-diamidino-2-phenylindole [DAPI]; blue) of adult sham, LSI, and aged mice injected with PBS or ABT263. Scale bar, 20 μm. ...View More

Pan, D., Benkato, K. G., Han, X., Zheng, J., Kumar, V., Wan, M., ... & Cao, X. (2024). Senescence of endplate osteoclasts induces sensory innervation and spinal pain. Elife, 12, RP92889.

Rat Anti-EMCN Recombinant Antibody (28) (CBMAB-E0280-FY) in IF

Type H vessels were labeled using immunofluorescence staining of CD31 and EMCN, followed by quantitative analysis of CD31 and EMCN-positive cells. Scale bar: 100 µm. ...View More

Li, S., Wu, H., Wang, F., Kong, L., Yu, Y., Zuo, R., ... & Kang, Q. (2024). Enhanced bone regeneration through regulation of mechanoresponsive FAK-ERK1/2 signaling by ZINC40099027 during distraction osteogenesis. International Journal of Medical Sciences, 21(1), 137.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

Application

WB, IP, IF, IHC-P, FC

Immunogen

Mouse bEND.3 endothelioma.

Host Species

Rat

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ENDOMUCIN

Introduction

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001).

Entrez Gene ID

59308

UniProt ID

Q9R0H2

Alternative Names

Muc14; AI315669; 0610012K22Rik

Research Area

Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

Biological Process

Hematopoietic stem cell homeostasis Source: Ensembl

Cellular Location

Isoform 1: Cell membrane; Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.
Isoform 2: Secreted

Topology

Extracellular: 19-190
Helical: 191-211
Cytoplasmic: 212-261

PTM

Highly O-glycosylated. Sialic acid-rich glycoprotein.

Huang, Q., Li, X. M., Sun, J. P., & Zhou, Y. (2022). Tumor‐derived endomucin promotes colorectal cancer proliferation and metastasis. Cancer Medicine.

Shen, Z., Dong, W., Chen, Z., Chen, G., Zhang, Y., Li, Z., ... & Jiang, Z. (2022). Total flavonoids of Rhizoma Drynariae enhances CD31 hi Emcn hi vessel formation and subsequent bone regeneration in rat models of distraction osteogenesis by activating PDGF‑BB/VEGF/RUNX2/OSX signaling axis. International Journal of Molecular Medicine, 50(3), 1-13.

Engelhard, S., Drilsvik, M., Gonzalez, F. R., Nerlov, C., Weischenfeldt, J., Reckzeh, K., & Theilgaard-Mönch, K. (2022). P1403: ENDOMUCIN IS A MARKER OF QUIESCENT HAEMATOPOIETIC STEM CELLS WITH HIGH LONG-TERM ENGRAFTMENT CAPACITY. HemaSphere, 6, 1287-1288.

Zhang, P., Yue, K., Liu, X., Yan, X., Yang, Z., Duan, J., ... & Han, H. (2020). Endothelial Notch activation promotes neutrophil transmigration via downregulating endomucin to aggravate hepatic ischemia/reperfusion injury. Science China Life Sciences, 63(3), 375-387.

Yang, J., LeBlanc, M. E., Cano, I., Saez-Torres, K. L., Saint-Geniez, M., Ng, Y. S., & D'Amore, P. A. (2020). ADAM10 and ADAM17 proteases mediate proinflammatory cytokine-induced and constitutive cleavage of endomucin from the endothelial surface. Journal of Biological Chemistry, 295(19), 6641-6651.

Yang, M., Li, C. J., Xiao, Y., Guo, Q., Huang, Y., Su, T., ... & Jiang, T. J. (2020). Ophiopogonin D promotes bone regeneration by stimulating CD31hiEMCNhi vessel formation. Cell proliferation, 53(3), e12784.

Hu, Z., Cano, I., Saez-Torres, K. L., LeBlanc, M. E., Saint-Geniez, M., Ng, Y. S., ... & D’Amore, P. A. (2020). Elements of the endomucin extracellular domain essential for VEGF-induced VEGFR2 activity. Cells, 9(6), 1413.

Niu, T., Zhao, M., Jiang, Y., Xing, X., Shi, X., Cheng, L., ... & Liu, K. (2019). Endomucin restores depleted endothelial glycocalyx in the retinas of streptozotocin‐induced diabetic rats. The FASEB Journal, 33(12), 13346-13357.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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