MSR1 Antibodies

Structure of MSR1

The protein encoded by the MSR1 gene has a molecular weight of approximately 75-80 kDa and is expressed by macrophages. This protein contains 451 amino acids and its secondary structure is mainly composed of α-helices and collagen-like domains, forming a characteristic trimeric helical structure. The protein structure of MSR1 contains a cysteine-rich domain, which can recognize various ligands through the coiled-coil structure, including acetylated low-density lipoprotein and pathogen surface molecules. The ligand-binding domain of this protein contains multiple positively charged amino acid residues, which can form electrostatic interactions with negatively charged ligands. The recombinant protein of its specific domain has been widely applied in the study of immune regulatory mechanisms.

Fig. 1 Classification of scavenger receptors and MSR1 isoforms.¹

Key structural properties of MSR1:

• Contains cysteine domain, collagen-like domain and helical coiled-coil domain
• The transmembrane region anchors to the macrophage membrane
• The trimeric structure forms the ligand binding site
• Positive charge amino acids mediate the binding with negatively charged ligands

Functions of MSR1

The scavenger receptor encoded by the MSR1 gene mainly functions to recognize and remove harmful substances in the body, and is involved in immune regulation and lipid metabolism.

The broad-spectrum recognition ability of MSR1 for various ligands enables it to play a dual role in atherosclerosis and infectious diseases, and its expression level is strictly regulated by the inflammatory microenvironment.

Applications of MSR1 and MSR1 Antibody in Literature

1. Gudgeon, Jack, José Luis Marín-Rubio, and Matthias Trost. "The role of macrophage scavenger receptor 1 (MSR1) in inflammatory disorders and cancer." Frontiers in immunology 13 (2022): 1012002. https://doi.org/10.3389/fimmu.2022.1012002

The research has found that the macrophage scavenger receptor 1 (MSR1/CD204) plays a dual role in atherosclerosis, immunity and cancer. This article reviews its expression regulation mechanism, the impact on macrophage function, the progress in signal pathway research, and the therapeutic potential in tumor-associated macrophages.

2. Zhao, Shu-Jie, et al. "Macrophage MSR1 promotes BMSC osteogenic differentiation and M2-like polarization by activating PI3K/AKT/GSK3β/β-catenin pathway." Theranostics 10.1 (2020): 17. https://doi.org/10.7150/thno.36930

The study found that macrophages with MSR1 regulate PGC1α through the PI3K/AKT/GSK3β/β-catenin pathway, promoting M2 polarization and mitochondrial oxidative phosphorylation, thereby inducing osteogenic differentiation of BMSCs and accelerating fracture healing. MSR1 may be a new target for fracture repair.

3. Jia, Jinchao, et al. "Ferritin triggers neutrophil extracellular trap-mediated cytokine storm through Msr1 contributing to adult-onset Still’s disease pathogenesis." Nature Communications 13.1 (2022): 6804. https://doi.org/10.1038/s41467-022-34560-7

The study found that ferritin induces the formation of NETs by upregulating and binding to neutrophil Msr1, thereby driving the inflammation of hyperferritinemia syndrome. Removing neutrophils or Msr1 can alleviate the damage. This mechanism has been confirmed in adult Still's disease, suggesting that Msr1 is a potential therapeutic target.

4. Nance, Sierra A., et al. "MSR1 is not required for obesity-associated inflammation and insulin resistance in mice." Scientific Reports 13.1 (2023): 2651. https://doi.org/10.1038/s41598-023-29736-0

The study found that MSR1 is highly expressed in the adipose tissue of obese humans and is associated with diabetes, and can serve as a biomarker. However, in the mouse model, regardless of the gene dosage, the absence of Msr1 did not improve obesity, insulin resistance, and macrophage accumulation, suggesting that it is not a key pathogenic gene in mice.

5. Sheng, Wei, Guang Ji, and Li Zhang. "Role of macrophage scavenger receptor MSR1 in the progression of non-alcoholic steatohepatitis." Frontiers in Immunology 13 (2022): 1050984. https://doi.org/10.3389/fimmu.2022.1050984

The research has found that in non-alcoholic steatohepatitis (NASH), lipid metabolism disorders lead to the generation of modified lipoproteins through oxidative stress. Macrophage scavenger receptor 1 (MSR1) takes in these modified lipoproteins to form foam cells, thereby promoting the progression of NASH. This article reviews the regulation of MSR1 expression and its role in the pathogenesis of NASH.

Creative Biolabs: MSR1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality MSR1 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom MSR1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MSR1 antibodies, custom preparations, or technical support, contact us at email.