Mouse Anti-AAV-5 Recombinant Antibody (V2-503417) (CBMAB-V208-1369-FY)

Name: Mouse Anti-AAV-5 Recombinant Antibody (V2-503417)
SKU: CBMAB-V208-1369-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AAV-5 Recombinant Antibody (CBMW-H1199) (CBMAB-V208-1369-FY) in Neutralize

The AAV vector (AAV5 [A and C] or AAV8 [B and D]) expressing secNanoLuc was incubated with an indicated concentration of human immunoglobulin (IVIG) (A and B) or anti-AAV monoclonal antibody (mAb) (C and D) for 1 h. CHO-K1 cells for AAV5 and Huh-7 cells for AAV8 were transduced with the AAV vector at an MOI of 100 (orange) or 1,000 (blue). Transgene expressions in the supernatant and cell lysate were determined by luminescence and expressed as transduction efficiency (%). Complete transduction (100%) was defined based on the result of the RLU obtained from incubation of the AAV vector with fetal bovine serum. Inhibition of vector transduction by neutralizing antibody is expressed as the percentage of transduction. ND50 values were calculated as the dilutions needed to neutralize 50% vector transduction. Values are presented as mean ± SD (n = 3). NTC, no transduction control. ...View More

Baatartsogt, N., Kashiwakura, Y., Hayakawa, M., Kamoshita, N., Hiramoto, T., Mizukami, H., & Ohmori, T. (2021). A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid. Molecular Therapy-Methods & Clinical Development, 22, 162-171.

Mouse Anti-AAV-5 Recombinant Antibody (CBMW-H1199) (CBMAB-V208-1369-FY) in IF

Capsid assembly of AAV2, -4, -5, -8, and -9 in HeLa cells. HeLa cells were transiently transfected with plasmids expressing VP3 proteins derived from AAV2,-4, -5, -8, or -9 in the presence (+) or absence (−) of a cotransfected plasmid expressing their cognate AAP. The groups that received no AAP-expressing plasmid were transfected with pCMV3-GFP instead to ensure successful transfection and maintain the total quantity of transfected DNA constant across the groups. The cells were fixed at 48 h posttransfection, immunostained with the antibodies indicated in each panel, and counterstained with DAPI. The images were obtained by using a Zeiss LSM 710 confocal microscope with a 63×/1.4-NA objective. Three representative fields of view are shown for each condition. (A) Cells were stained with anti-AAV capsid antibody (A20 for AAV2, ADK4 for AAV4, ADK5a for AAV5, ADK8 for AAV8, and ADK8/9 for AAV9) (red) and anti-FLAG antibody (white). Bar, 10 μm. ...View More

Earley, L. F., Powers, J. M., Adachi, K., Baumgart, J. T., Meyer, N. L., Xie, Q., ... & Nakai, H. (2017). Adeno-associated virus (AAV) assembly-activating protein is not an essential requirement for capsid assembly of AAV serotypes 4, 5, and 11. Journal of virology, 91(3), e01980-16.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-503417

Application

ELISA, IHC

Immunogen

AAV5 capsids

Specificity

AAV-5

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500
IF(ICC)	1:20
IP	1:5
Dot Blot	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adeno-Associated Virus-5

Introduction

Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease.

Alternative Names

AAV-5; Adeno-Associated Virus-5

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-AAV-5 Recombinant Antibody (V2-503416) (CAT#: CBMAB-V208-1402-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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