PFKL

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]

Full Name

phosphofructokinase, liver

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583).
Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).

Biological Process

Canonical glycolysisManual Assertion Based On ExperimentIBA:GO_Central
Fructose 1,6-bisphosphate metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Fructose 6-phosphate metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Glycolytic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of insulin secretionIEA:Ensembl
Response to glucoseManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm

PTM

GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.