Mouse Anti-PFKL Recombinant Antibody (2A9) (CBMAB-P1511-YC)

Name: Mouse Anti-PFKL Recombinant Antibody (2A9)
SKU: CBMAB-P1511-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2A9

Application

ELISA, WB

Immunogen

PFKL AA 441-540 partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

phosphofructokinase, liver

Introduction

PFKL is the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues.

Entrez Gene ID

5211

UniProt ID

P17858

Alternative Names

Phosphofructokinase, Liver Type; Phosphofructo-1-Kinase Isozyme B;-Phosphofructokinase Type B; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-B;

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583).
Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).

Biological Process

Canonical glycolysisManual Assertion Based On ExperimentIBA:GO_Central
Fructose 1,6-bisphosphate metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Fructose 6-phosphate metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Glycolytic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of insulin secretionIEA:Ensembl
Response to glucoseManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm

PTM

GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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