UBE2W

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.

Full Name

ubiquitin-conjugating enzyme E2W (putative)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:20061386, PubMed:21229326).
Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:23560854, PubMed:23696636, PubMed:25436519).
Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:19111657, PubMed:21229326).
In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (PubMed:25436519).

Biological Process

Biological Process cellular response to misfolded protein Source:UniProtKB
Biological Process DNA repair Source:UniProtKB-KW
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB2 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process protein quality control for misfolded or incompletely synthesized proteins Source:UniProtKB

Cellular Location

Nucleus
In the nucleus, colocalizes with FANCL.

PTM

Autoubiquitinated at Met-1.