Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) (CBMAB-A3547-YC)

Name: Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766)
SKU: CBMAB-A3547-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in IP

Immunoblots of BRG1, SNF5, ARID2, PBRM1, and ARID1A in SKmel147 ARID2 WT and KO cells following immunoprecipitation of BRG1. IgG was used as negative control (n = 2). ...View More

Carcamo, S., Nguyen, C. B., Grossi, E., Filipescu, D., Alpsoy, A., Dhiman, A., ... & Bernstein, E. (2022). Altered BAF occupancy and transcription factor dynamics in PBAF-deficient melanoma. Cell reports, 39(1).

Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in IF

Representative image using deconvolution microscopy of cells stained with antibodies directed against BRG1 (red, upper two panels) or ARID2 (red, lower two panels) and -tubulin (green), DAPI to visualize chromosomes (blue). Representative images show BRG1 and ARID2 localization at the mitotic spindle and spindle pole in PBRM1-proficient HEK293T cells (upper panel) and loss of mitotic spindle localization
in PBRM1-depleted HEK293T cells (lower panel). Scale bars, 5 m (n = 3 biological replicates). ...View More

Karki, M., Jangid, R. K., Anish, R., Seervai, R. N., Bertocchio, J. P., Hotta, T., ... & Tripathi, D. N. (2021). A cytoskeletal function for PBRM1 reading methylated microtubules. Science advances, 7(14), eabf2866.

Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in Immunoblot

IB analysis showing coimmunoprecipitation of PBRM1 and tubulin, coimmunoprecipitation of BRG1 and tubulin, and their corresponding input lysates from PBRM1-proficient, PBRM1-depleted, and PBRM1-rescued HEK293T cells showing loss of interaction between BRG1 and tubulin with loss of PBRM1. Representative blots (n = 3). ...View More

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A766

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1134-1316 mapping within an internal region of ARID2 of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ARID2

Introduction

ARID2 is a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin

Entrez Gene ID

Human	196528
Mouse	77044
Rat	366980

UniProt ID

Human	Q68CP9
Mouse	E9Q7E2
Rat	D3ZJU0

Alternative Names

AT-Rich Interaction Domain 2; AT Rich Interactive Domain 2 (ARID, RFX-Like); Zinc Finger Protein With Activation Potential; ARID Domain-Containing Protein 2; BRG1-Associated Factor 200; Zipzap/P200;

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.

Biological Process

Cardiac muscle cell proliferation Source: Ensembl
Coronary artery morphogenesis Source: Ensembl
Embryonic organ development Source: Ensembl
Heart morphogenesis Source: Ensembl
Homeostatic process Source: Ensembl
Negative regulation of cell migration Source: BHF-UCL
Negative regulation of cell population proliferation Source: BHF-UCL
Nucleosome disassembly Source: BHF-UCL
Positive regulation of double-strand break repair via homologous recombination Source: CACAO
Regulation of transcription, DNA-templated Source: InterPro

Cellular Location

Nucleus

Involvement in disease

Coffin-Siris syndrome 6 (CSS6): A form of Coffin-Siris syndrome, a congenital multiple malformation syndrome with broad phenotypic and genetic variability. Cardinal features are intellectual disability, coarse facial features, hypertrichosis, and hypoplastic or absent fifth digit nails or phalanges. Additional features include malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems. Sucking/feeding difficulties, poor growth, ophthalmologic abnormalities, hearing impairment, and spinal anomalies are common findings. Both autosomal dominant and autosomal recessive inheritance patterns have been reported. CSS6 inheritance is autosomal dominant.

Liu, X., Zhang, H., Zhou, P., Yu, Y., Zhang, H., Chen, L., ... & Liu, Z. (2021). CREB1 acts via the miR‑922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncology reports, 45(5), 1-13.

Wu, M., Duan, Q., Liu, X., Zhang, P., Fu, Y., Zhang, Z., ... & Jiang, H. (2020). MiR-155-5p promotes oral cancer progression by targeting chromatin remodeling gene ARID2. Biomedicine & Pharmacotherapy, 122, 109696.

Loesch, R., Chenane, L., & Colnot, S. (2020). ARID2 Chromatin Remodeler in Hepatocellular Carcinoma. Cells, 9(10), 2152.

Yang, Y. L., Hu, F., Xue, M., Jia, Y. J., Zheng, Z. J., Li, Y., & Xue, Y. M. (2019). Early growth response protein-1 upregulates long noncoding RNA Arid2-IR to promote extracellular matrix production in diabetic kidney disease. American Journal of Physiology-Cell Physiology, 316(3), C340-C352.

Gazdagh, G., Blyth, M., Scurr, I., Turnpenny, P. D., Mehta, S. G., Armstrong, R., ... & Joss, S. (2019). Extending the clinical and genetic spectrum of ARID2 related intellectual disability. A case series of 7 patients. European journal of medical genetics, 62(1), 27-34.

Wang, Y., Chang, W., Chang, W., Chang, X., Zhai, S., Pan, G., & Dang, S. (2018). MicroRNA-376c-3p facilitates human hepatocellular carcinoma progression via repressing AT-rich interaction domain 2. Journal of Cancer, 9(22), 4187.

Tao, B., Yi, J., Huang, C., Xu, W., Qin, C., Chen, L., ... & Wang, R. (2017). microRNA‑96 regulates the proliferation of nucleus pulposus cells by targeting ARID2/AKT signaling. Molecular medicine reports, 16(5), 7553-7560.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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