Sign in or Register   Sign in or Register
  |  

Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) (CBMAB-A3547-YC)

Provided herein is a Mouse monoclonal antibody against Human AT-Rich Interaction Domain 2. The antibody can be used for immunoassay techniques, such as Dot, ICC, WB.
See all ARID2 antibodies
Published Data
Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in IP
Immunoblots of BRG1, SNF5, ARID2, PBRM1, and ARID1A in SKmel147 ARID2 WT and KO cells following immunoprecipitation of BRG1. IgG was used as negative control (n = 2). ...View More
Carcamo, S., Nguyen, C. B., Grossi, E., Filipescu, D., Alpsoy, A., Dhiman, A., ... & Bernstein, E. (2022). Altered BAF occupancy and transcription factor dynamics in PBAF-deficient melanoma. Cell reports, 39(1).
Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in IF
Representative image using deconvolution microscopy of cells stained with antibodies directed against BRG1 (red, upper two panels) or ARID2 (red, lower two panels) and -tubulin (green), DAPI to visualize chromosomes (blue). Representative images show BRG1 and ARID2 localization at the mitotic spindle and spindle pole in PBRM1-proficient HEK293T cells (upper panel) and loss of mitotic spindle localization
in PBRM1-depleted HEK293T cells (lower panel). Scale bars, 5 m (n = 3 biological replicates). ...View More
Karki, M., Jangid, R. K., Anish, R., Seervai, R. N., Bertocchio, J. P., Hotta, T., ... & Tripathi, D. N. (2021). A cytoskeletal function for PBRM1 reading methylated microtubules. Science advances, 7(14), eabf2866.
Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766) CBMAB-A3547-YC in Immunoblot
IB analysis showing coimmunoprecipitation of PBRM1 and tubulin, coimmunoprecipitation of BRG1 and tubulin, and their corresponding input lysates from PBRM1-proficient, PBRM1-depleted, and PBRM1-rescued HEK293T cells showing loss of interaction between BRG1 and tubulin with loss of PBRM1. Representative blots (n = 3). ...View More
Karki, M., Jangid, R. K., Anish, R., Seervai, R. N., Bertocchio, J. P., Hotta, T., ... & Tripathi, D. N. (2021). A cytoskeletal function for PBRM1 reading methylated microtubules. Science advances, 7(14), eabf2866.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A766
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1134-1316 mapping within an internal region of ARID2 of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ARID2
Introduction
ARID2 is a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin
Entrez Gene ID
Human196528
Mouse77044
Rat366980
UniProt ID
HumanQ68CP9
MouseE9Q7E2
RatD3ZJU0
Alternative Names
AT-Rich Interaction Domain 2; AT Rich Interactive Domain 2 (ARID, RFX-Like); Zinc Finger Protein With Activation Potential; ARID Domain-Containing Protein 2; BRG1-Associated Factor 200; Zipzap/P200;
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
Biological Process
Cardiac muscle cell proliferation Source: Ensembl
Coronary artery morphogenesis Source: Ensembl
Embryonic organ development Source: Ensembl
Heart morphogenesis Source: Ensembl
Homeostatic process Source: Ensembl
Negative regulation of cell migration Source: BHF-UCL
Negative regulation of cell population proliferation Source: BHF-UCL
Nucleosome disassembly Source: BHF-UCL
Positive regulation of double-strand break repair via homologous recombination Source: CACAO
Regulation of transcription, DNA-templated Source: InterPro
Cellular Location
Nucleus
Involvement in disease
Coffin-Siris syndrome 6 (CSS6): A form of Coffin-Siris syndrome, a congenital multiple malformation syndrome with broad phenotypic and genetic variability. Cardinal features are intellectual disability, coarse facial features, hypertrichosis, and hypoplastic or absent fifth digit nails or phalanges. Additional features include malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems. Sucking/feeding difficulties, poor growth, ophthalmologic abnormalities, hearing impairment, and spinal anomalies are common findings. Both autosomal dominant and autosomal recessive inheritance patterns have been reported. CSS6 inheritance is autosomal dominant.

Liu, X., Zhang, H., Zhou, P., Yu, Y., Zhang, H., Chen, L., ... & Liu, Z. (2021). CREB1 acts via the miR‑922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncology reports, 45(5), 1-13.

Wu, M., Duan, Q., Liu, X., Zhang, P., Fu, Y., Zhang, Z., ... & Jiang, H. (2020). MiR-155-5p promotes oral cancer progression by targeting chromatin remodeling gene ARID2. Biomedicine & Pharmacotherapy, 122, 109696.

Loesch, R., Chenane, L., & Colnot, S. (2020). ARID2 Chromatin Remodeler in Hepatocellular Carcinoma. Cells, 9(10), 2152.

Yang, Y. L., Hu, F., Xue, M., Jia, Y. J., Zheng, Z. J., Li, Y., & Xue, Y. M. (2019). Early growth response protein-1 upregulates long noncoding RNA Arid2-IR to promote extracellular matrix production in diabetic kidney disease. American Journal of Physiology-Cell Physiology, 316(3), C340-C352.

Gazdagh, G., Blyth, M., Scurr, I., Turnpenny, P. D., Mehta, S. G., Armstrong, R., ... & Joss, S. (2019). Extending the clinical and genetic spectrum of ARID2 related intellectual disability. A case series of 7 patients. European journal of medical genetics, 62(1), 27-34.

Wang, Y., Chang, W., Chang, W., Chang, X., Zhai, S., Pan, G., & Dang, S. (2018). MicroRNA-376c-3p facilitates human hepatocellular carcinoma progression via repressing AT-rich interaction domain 2. Journal of Cancer, 9(22), 4187.

Tao, B., Yi, J., Huang, C., Xu, W., Qin, C., Chen, L., ... & Wang, R. (2017). microRNA‑96 regulates the proliferation of nucleus pulposus cells by targeting ARID2/AKT signaling. Molecular medicine reports, 16(5), 7553-7560.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ARID2 Recombinant Antibody (CBYC-A766)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare