Mouse Anti-ATAD1 Recombinant Antibody (S125-10) (CBMAB-A3832-YC)

Name: Mouse Anti-ATAD1 Recombinant Antibody (S125-10)
SKU: CBMAB-A3832-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

S125-10

Application

ICC, IF, IHC, WB

Immunogen

Fusion protein amino acids 1-361 (Full length) of mouse Thorase.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IHC	1:1,000
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% glycerol

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-361

More Infomation

Target

Full Name

ATPase Family, AAA Domain Containing 1

Introduction

ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recyclin

Entrez Gene ID

67979

UniProt ID

Q9D5T0

Alternative Names

ATPase Family, AAA Domain Containing 1; Thorase; ATPase Family AAA Domain-Containing Protein 1; EC 3.6.1.3; FNP001; AFDC1;

Function

Outer mitochondrial translocase required to remove mislocalized tail-anchored transmembrane proteins on mitochondria (PubMed:24843043).
Specifically recognizes and binds tail-anchored transmembrane proteins: acts as a dislocase that mediates the ATP-dependent extraction of mistargeted tail-anchored transmembrane proteins from the mitochondrion outer membrane (By similarity).
Also plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory (By similarity).
Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent (By similarity).

Biological Process

Extraction of mislocalized protein from mitochondrial outer membrane Source: UniProtKB
Learning Source: UniProtKB
Memory Source: UniProtKB
Negative regulation of synaptic transmission, glutamatergic Source: UniProtKB
Positive regulation of receptor internalization Source: UniProtKB
Protein import into peroxisome membrane Source: Reactome
Regulation of postsynaptic neurotransmitter receptor internalization Source: Ensembl

Cellular Location

Mitochondrion outer membrane; Postsynaptic cell membrane; Peroxisome membrane

Involvement in disease

Hyperekplexia 4 (HKPX4): An autosomal recessive severe neurologic disorder apparent from birth. HKPX4 is characterized by little if any development, hypertonia, early-onset refractory seizures in some patients, and respiratory failure resulting in early death, mostly in the first months of life.

Topology

Mitochondrial intermembrane: 1-15 aa
Helical: 16-32 aa
Cytoplasmic: 33-361 aa

Wang, L., & Walter, P. (2020). Msp1/ATAD1 in protein quality control and regulation of synaptic activities. Annual Review of Cell and Developmental Biology, 36, 141-164.

Piard, J., Umanah, G. K. E., Harms, F. L., Abalde-Atristain, L., Amram, D., Chang, M., ... & Kutsche, K. (2018). A homozygous ATAD1 mutation impairs postsynaptic AMPA receptor trafficking and causes a lethal encephalopathy. Brain, 141(3), 651-661.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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