Mouse Anti-BRD8 Recombinant Antibody (CBYY-0808) (CBMAB-0811-YY)

This product is mouse antibody that recognizes BRD8. The antibody CBYY-0808 can be used for immunoassay techniques such as: ELISA, ICC, IF
See all BRD8 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYY-0808

Antibody Isotype

IgG2b, κ

Application

ELISA, ICC, IF

Basic Information

Immunogen

BRD8 (NP_631938, 33 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.2-1.0 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Bromodomain Containing 8

Introduction

The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforMouse have been identified. [provided by RefSeq, Jul 2014]

Entrez Gene ID

10902

UniProt ID

Q9H0E9

Alternative Names

Bromodomain Containing 8; Thyroid Hormone Receptor Coactivating Protein Of 120 KDa; Skeletal Muscle Abundant Protein 2; TrCP120; SMAP2; SMAP; P120; Bromodomain-Containing Protein 8; Skeletal Muscle Abundant Protein;

Function

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Cellular response to thyroid hormone stimulus Source: ARUK-UCL
Histone acetylation Source: GO_Central
Histone H2A acetylation Source: UniProtKB
Histone H4 acetylation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
Regulation of growth Source: UniProtKB-KW

Cellular Location

Nucleus

References

Browne, J. A., NandyMazumdar, M., Paranjapye, A., Leir, S. H., & Harris, A. (2021). The Bromodomain Containing 8 (BRD8) transcriptional network in human lung epithelial cells. Molecular and Cellular Endocrinology, 524, 111169.

Remillard, D., Savage, N. A., Kedves, A. T., Paulk, J., Chen, X., Garcia, F. J., ... & Forrester, W. C. (2021). Chemoproteomics Enabled Discovery of Selective Probes for NuA4 Factor BRD8. bioRxiv.

Yu, Z., Chen, T., Mo, H., Guo, C., & Liu, Q. (2020). BRD8, which is negatively regulated by miR-876–3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5. Archives of Biochemistry and Biophysics, 693, 108550.

Chen, Y. R., Ouyang, S. S., Chen, Y. L., Li, P., Xu, H. W., & Zhu, S. L. (2020). BRD4/8/9 are prognostic biomarkers and associated with immune infiltrates in hepatocellular carcinoma. Aging (Albany NY), 12(17), 17541.

Lashgari, A., Fauteux, M., Maréchal, A., & Gaudreau, L. (2018). Cellular depletion of BRD8 causes p53-dependent apoptosis and induces a DNA damage response in non-stressed cells. Scientific reports, 8(1), 1-12.

Micci, F., Brunetti, M., Dal Cin, P., Nucci, M. R., Gorunova, L., Heim, S., & Panagopoulos, I. (2017). Fusion of the genes BRD8 and PHF1 in endometrial stromal sarcoma. Genes, Chromosomes and Cancer, 56(12), 841-845.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-BRD8 Recombinant Antibody (3G8)

Mouse Anti-BRD8 Recombinant Antibody (407)

Mouse Anti-BRD8 Recombinant Antibody (CF054)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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