Mouse Anti-CENPJ Recombinant Antibody (CBLNC-249) (CBMAB-1353-CN)

Name: Mouse Anti-CENPJ Recombinant Antibody (CBLNC-249)
SKU: CBMAB-1353-CN
Rating: 5 (1 reviews)

See all CENPJ antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNC-249

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 1087-1338 of human CENPJ.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centromere Protein J

Introduction

Centromere protein J (CENPJ) belongs to the centromere protein family and plays a structural role in the maintenance of centrosome integrity and normal spindle morphology. CENPJ can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability.

Entrez Gene ID

55835

UniProt ID

Q9HC77

Alternative Names

LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J

Function

Plays an important role in cell division and centrosome function by participating in centriole duplication (PubMed:17681131, PubMed:20531387).
Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles (PubMed:15047868, PubMed:27219064, PubMed:27306797).
Required for centriole elongation and for STIL-mediated centriole amplification (PubMed:22020124).
Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (PubMed:27185865).
May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release (PubMed:27306797).

Biological Process

Astral microtubule nucleation Source: MGI
Cell division Source: UniProtKB
Centriole elongation Source: UniProtKB
Centriole replication Source: UniProtKB
Cliary basal body-plasma membrane docking Source: Reactome
Cilium assembly Source: GO_Central
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule nucleation Source: UniProtKB
Microtubule polymerization Source: UniProtKB
Positive regulation of centriole elongation Source: UniProtKB
Positive regulation of establishment of protein localization Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: MGI
Regulation of centriole replication Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome

Cellular Location

Centrosome; Centriole. Localized within the center of microtubule asters (PubMed:11003675). During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles (PubMed:17681131).

Involvement in disease

Microcephaly 6, primary, autosomal recessive (MCPH6): A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder.
Seckel syndrome 4 (SCKL4): A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.

PTM

Phosphorylation at Ser-589 and Ser-595 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-595 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly.

Vijayan, K., Arang, N., Wei, L., Morrison, R., Geiger, R., Parks, R. K., ... & Kaushansky, A. (2021). A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection. bioRxiv, 2020-08.

Cueto-González, A. M., Fernández-Cancio, M., Fernández-Alvarez, P., García-Arumí, E., & Tizzano, E. F. (2020). Unusual context of CENPJ variants and primary microcephaly: compound heterozygosity and nonconsanguinity in an Argentinian patient. Human genome variation, 7(1), 1-5.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CENPJ Recombinant Antibody (CBLNC-249)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CENPJ Matched Antibody Pair (256) (CAT#: APMAB-256LY)

Related Products

Mouse Anti-CENPJ Recombinant Antibody (CBFYC-1717) (CAT#: CBMAB-C1779-FY)

Mouse Anti-CENPJ Monoclonal Antibody (1A5) (CAT#: CBMAB-0967-YC)

CENPJ Matched Antibody Pair (256) (CAT#: APMAB-256LY)

Mouse Anti-CENPJ Recombinant Antibody (CBXC-0367) (CAT#: CBMAB-C5760-CQ)

Mouse Anti-CENPJ Recombinant Antibody (5D5) (CAT#: CBMAB-1352-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry