Rabbit Anti-CCP110 Recombinant Antibody (EG644) (CBMAB-EN777-LY)

Name: Rabbit Anti-CCP110 Recombinant Antibody (EG644)
SKU: CBMAB-EN777-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG644

Application

WB, IHC-P

Immunogen

Recombinant fragmemt of human CP110.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Centriolar Coiled-Coil Protein 110

Entrez Gene ID

9738

UniProt ID

Q66GS9

Function

Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:17719545 PubMed:17681131, PubMed:23486064).
Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity).
Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed:16760425).

Biological Process

Centriole replication Source: UniProtKB
Centrosome duplication Source: UniProtKB
Ciliary basal body organization Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Negative regulation of centriole elongation Source: GO_Central
Negative regulation of cilium assembly Source: UniProtKB
Positive regulation of cilium assembly Source: UniProtKB
Protein deubiquitination Source: Reactome
Regulation of cytokinesis Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome

Cellular Location

Centriole; Centrosome; Cilium basal body. Recruited early and then associates with the growing distal tips. Recruited to the mother centriole by KIF24. Removed from centrioles by TTBK2, leading to initiation of ciliogenesis and localizes only to the daughter centriole in ciliated cells. In cytotoxic T lymphocytes remains associated with the mother centriole during docking of the centrosome at the immunological synapse upon target contact.

PTM

Phosphorylated by CDKs.
Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate.

Liu, M., Zhang, W., Li, M., Feng, J., Kuang, W., Chen, X., ... & Xie, S. (2021). NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. Cell research, 1-13.

Liu, J., Li, Y. Y., Ong, H. H., Tan, K. S., He, T. T., Thong, K. T., & Ong, Y. K. (2019). IL-13 regulates cilia loss and localization of cp110 in human nasal epithelium.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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