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Rabbit Anti-COPB2 Recombinant Antibody (1414) (V2LY-1206-LY755)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
1414
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Recombinant fusion protein amino acids 657-906 of human COPB2.
Host Species
Rabbit
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:3,000-1:20,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 0.05% BSA, 50% glycerol
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Coatomer Protein Complex Subunit Beta 2
Entrez Gene ID
Human9276
UniProt ID
HumanP35606
Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).

This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner (By similarity).
Biological Process
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Intracellular protein transport Source: GO_Central
Intra-Golgi vesicle-mediated transport Source: UniProtKB
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central
Toxin transport Source: Ensembl
Cellular Location
Cytosol; Golgi apparatus membrane; COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Shows only a slight preference for the cis-Golgi apparatus, compared with the trans-Golgi.
Involvement in disease
Microcephaly 19, primary, autosomal recessive (MCPH19):
A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age, sex and ethnically matched mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. MCPH19 affected individuals manifest severe developmental delay, failure to thrive, cortical blindness, and spasticity. Brain imaging show a simplified gyral pattern, thin corpus callosum, slight ventricular dilation, and delayed myelination.
More Infomation

Feng, Y., Lei, X., Zhang, L., Wan, H., Pan, H., Wu, J., ... & Mi, Y. (2021). COPB2: a transport protein with multifaceted roles in cancer development and progression. Clinical and Translational Oncology, 23(11), 2195-2205.

Marom, R., Burrage, L. C., Venditti, R., Clément, A., Blanco-Sánchez, B., Jain, M., ... & Lee, B. (2021). COPB2 loss of function causes a coatopathy with osteoporosis and developmental delay. The American Journal of Human Genetics, 108(9), 1710-1724.

Wu, B., Wu, Y., Guo, X., Yue, Y., Li, Y., He, X., ... & Zhang, S. (2021). An Integrative Pan-Cancer Analysis of the Oncogenic Role of COPB2 in Human Tumors. BioMed research international, 2021.

Zhang, J., Wang, X., Li, G., He, J., Lu, Z., Yang, Y., ... & Liu, J. (2021). COPB2: a novel prognostic biomarker that affects progression of HCC. BioMed research international, 2021.

Wang, Y., Xie, G., Li, M., Du, J., & Wang, M. (2020). COPB2 gene silencing inhibits colorectal cancer cell proliferation and induces apoptosis via the JNK/c-Jun signaling pathway. PloS one, 15(11), e0240106.

Bhandari, A., Zheng, C., Sindan, N., Sindan, N., Quan, R., Xia, E., ... & Huang, D. (2019). COPB2 is up‐regulated in breast cancer and plays a vital role in the metastasis via N‐cadherin and Vimentin. Journal of Cellular and Molecular Medicine, 23(8), 5235-5245.

Mi, Y., Sun, C., Wei, B., Sun, F., Guo, Y., Hu, Q., ... & Xia, G. (2018). Coatomer subunit beta 2 (COPB2), identified by label-free quantitative proteomics, regulates cell proliferation and apoptosis in human prostate carcinoma cells. Biochemical and biophysical research communications, 495(1), 473-480.

Li, Z. S., Liu, C. H., Liu, Z., Zhu, C. L., & Huang, Q. (2018). Downregulation of COPB2 by RNAi inhibits growth of human cholangiocellular carcinoma cells. Eur Rev Med Pharmacol Sci, 22(4), 985-992.

Wang, Y., Chai, Z., Wang, M., Jin, Y., Yang, A., & Li, M. (2018). COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle‑related proteins. Experimental and Therapeutic Medicine, 15(1), 777-784.

DiStasio, A., Driver, A., Sund, K., Donlin, M., Muraleedharan, R. M., Pooya, S., ... & Stottmann, R. W. (2017). Copb2 is essential for embryogenesis and hypomorphic mutations cause human microcephaly. Human molecular genetics, 26(24), 4836-4848.

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For research use only. Not intended for any clinical use.

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