Mouse Anti-CPPED1 Recombinant Antibody (CBLC178-LY) (V2LY-1206-LY978)

Name: Mouse Anti-CPPED1 Recombinant Antibody (CBLC178-LY)
SKU: V2LY-1206-LY978
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC178-LY

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 47-71 within an internal region of human CPPED1.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Calcineurin Like Phosphoesterase Domain Containing 1

Entrez Gene ID

Human	55313
Mouse	223978
Rat	302890

UniProt ID

Human	Q9BRF8
Mouse	Q8BFS6
Rat	Q66H71

Function

Protein phosphatase that dephosphorylates AKT family kinase specifically at 'Ser-473', blocking cell cycle progression and promoting cell apoptosis. May play an inhibitory role in glucose uptake by adipocytes.

Biological Process

Neutrophil degranulation Source: Reactome

Cellular Location

Cytoplasm

Haapalainen, A. M., Daddali, R., Hallman, M., & Rämet, M. (2021). Human CPPED1 belongs to calcineurin‐like metallophosphoesterase superfamily and dephosphorylates PI3K‐AKT pathway component PAK4. Journal of cellular and molecular medicine, 25(13), 6304-6317.

Daddali, R., Ojaniemi, M., Hallman, M., Rämet, M., & Haapalainen, A. M. (2020). CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. Plos one, 15(6), e0234403.

Haapalainen, A. M., Karjalainen, M. K., Daddali, R., Ohlmeier, S., Anttonen, J., Määttä, T. A., ... & Rämet, M. (2018). Expression of CPPED 1 in human trophoblasts is associated with timing of term birth. Journal of cellular and molecular medicine, 22(2), 968-981.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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