Mouse Anti-DNAJB11 Recombinant Antibody (EG976) (V2LY-0425-LY629)

Name: Mouse Anti-DNAJB11 Recombinant Antibody (EG976)
SKU: V2LY-0425-LY629
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG976

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 106-235 mapping within an internal region of human ERdj3.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member B11

Entrez Gene ID

Human	51726
Mouse	67838
Rat	360734

UniProt ID

Human	Q9UBS4
Mouse	Q99KV1
Rat	Q6TUG0

Function

As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins (PubMed:10827079, PubMed:15525676, PubMed:29706351).

Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed (PubMed:15525676).

May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (PubMed:10827079).

It is necessary for maturation and correct trafficking of PKD1 (PubMed:29706351).

Biological Process

Positive regulation of ATPase activity Source: AgBase
Protein folding Source: InterPro
Protein maturation Source: UniProtKB

Cellular Location

Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.

Involvement in disease

Polycystic kidney disease 6 with or without polycystic liver disease (PKD6):
A form of polycystic kidney disease, a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occur in other organs, particularly the liver. PKD6 inheritance is autosomal dominant.

PTM

Contains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.

Pisani, I., Allinovi, M., Palazzo, V., Zanelli, P., Gentile, M., Farina, M. T., ... & Manenti, L. (2022). More dissimilarities than affinities between DNAJB11-PKD and ADPKD. Clinical Kidney Journal.

Jordan, P., Arrondel, C., Bessières, B., Tessier, A., Attié-Bitach, T., Guterman, S., ... & Heidet, L. (2021). Bi-allelic pathogenic variations in DNAJB11 cause Ivemark II syndrome, a renal-hepatic-pancreatic dysplasia. Kidney International, 99(2), 405-409.

Sun, R., Yang, L., Wang, Y., Zhang, Y., Ke, J., & Zhao, D. (2021). DNAJB11 predicts a poor prognosis and is associated with immune infiltration in thyroid carcinoma: a bioinformatics analysis. Journal of International Medical Research, 49(11), 03000605211053722.

Huynh, V. T., Audrézet, M. P., Sayer, J. A., Ong, A. C., Lefevre, S., Le Brun, V., ... & Genomics England Research Consortium. (2020). Clinical spectrum, prognosis and estimated prevalence of DNAJB11-kidney disease. Kidney international, 98(2), 476-487.

Wilson, G. J., Wood, S., Patel, C., Oliver, K., John, G., Ranganathan, D., ... & Isbel, N. (2020). DNAJB11-related atypical ADPKD in a kidney transplant donor. Kidney international reports, 5(8), 1363-1366.

Pan, J., Cao, D., & Gong, J. (2018). The endoplasmic reticulum co-chaperone ERdj3/DNAJB11 promotes hepatocellular carcinoma progression through suppressing AATZ degradation. Future Oncology, 14(29), 3001-3013.

Cornec-Le Gall, E., Olson, R. J., Besse, W., Heyer, C. M., Gainullin, V. G., Smith, J. M., ... & Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease. (2018). Monoallelic mutations to DNAJB11 cause atypical autosomal-dominant polycystic kidney disease. The American Journal of Human Genetics, 102(5), 832-844.

Allison, S. J. (2018). DNAJB11: another player in ADPKD. Nature Reviews Nephrology, 14(8), 476-476.

Chen, K. C., Qu, S., Chowdhury, S., Noxon, I. C., Schonhoft, J. D., Plate, L., ... & Wiseman, R. L. (2017). The endoplasmic reticulum HSP 40 co‐chaperone ER dj3/DNAJB 11 assembles and functions as a tetramer. The EMBO journal, 36(15), 2296-2309.

Guorong, Y., Yunfeng, F. U., Yanli, L. I., Caiyun, Z., & Weiguo, L. V. (2017). Expression and clinical significance of DNAJB11 in epithelial ovarian cancer. Zhejiang da xue xue bao. Yi xue ban= Journal of Zhejiang University. Medical Sciences, 46(2), 173-178.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-DNAJB11 Recombinant Antibody (EG976) (CAT#: CBMAB-EN1158-LY)

Related Products

Rabbit Anti-DNAJB11 Recombinant Antibody (EG976) (CAT#: CBMAB-EN1158-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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