Mouse Anti-DOK7 Recombinant Antibody (5G8) (V2LY-0425-LY800)

Name: Mouse Anti-DOK7 Recombinant Antibody (5G8)
SKU: V2LY-0425-LY800
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5G8

Application

FC, IF, IHC, WB

Immunogen

Full length human recombinant protein of human DOK7 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:100
IHC	1:150
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Docking Protein 7

Entrez Gene ID

285489

UniProt ID

Q18PE1

Function

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Biological Process

Neuromuscular junction development Source: GO_Central
Positive regulation of protein tyrosine kinase activity Source: UniProtKB

Cellular Location

Cell membrane; Synapse. Accumulates at neuromuscular junctions.

Involvement in disease

Myasthenic syndrome, congenital, 10 (CMS10):
A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. CMS10 is an autosomal recessive, post-synaptic form characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
Fetal akinesia deformation sequence 3 (FADS3):
A clinically and genetically heterogeneous group of disorders with congenital malformations related to impaired fetal movement. Clinical features include fetal akinesia, intrauterine growth retardation, polyhydramnios, arthrogryposis, pulmonary hypoplasia, craniofacial abnormalities, and cryptorchidism. FADS3 inheritance is autosomal recessive.

Bastos, P., Barbosa, R., Fernandes, M., & Alonso, I. (2020). A late-onset congenital myasthenic syndrome due to a heterozygous DOK7 mutation. Neuromuscular Disorders, 30(4), 331-335.

Kim, S. S., Park, M., & Park, S. (2020). Replicated Association Study between Tuberculosis and CLCN6, DOK7, HLA-DRA in Korean. Biomedical Science Letters, 26(3), 238-243.

Hua, C. D., Bian, E. B., Chen, E. F., Yang, Z. H., Tang, F., Wang, H. L., & Zhao, B. (2018). Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation. Biomedicine & pharmacotherapy, 106, 678-685.

Yang, S. M., Li, S. Y., Yu, H. B., Li, J. R., & Sun, L. L. (2017). Repression of DOK7 mediated by DNMT3A promotes the proliferation and invasion of KYSE410 and TE-12 ESCC cells. Biomedicine & Pharmacotherapy, 90, 93-99.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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