Mouse Anti-GSTM3 Recombinant Antibody (
2H9D7) (V2LY-0725-LY153)

Name: Mouse Anti-GSTM3 Recombinant Antibody (2H9D7)
SKU: V2LY-0725-LY153
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-GSTM3 Recombinant Antibody (2H9D7) (V2LY-0725-LY153) in WB

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Rat brain tissue All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 27 KDa Observed band size: 27 KDa Exposure time:10min

Mouse Anti-GSTM3 Recombinant Antibody (2H9D7) (V2LY-0725-LY153) in IHC

IHC image of V2LY-0725-LY153 diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse Anti-GSTM3 Recombinant Antibody (2H9D7) (V2LY-0725-LY153) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY153 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2H9D7

Application

ELISA, WB, IHC, FC

Immunogen

Recombinant Human Glutathione S-transferase Mu 3 protein (1-225AA).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:5,000
IHC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

GSTM3

Entrez Gene ID

2947

UniProt ID

P21266

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

Biological Process

Cellular detoxification of nitrogen compound Source: BHF-UCL
Establishment of blood-nerve barrier Source: ProtInc
Glutathione metabolic process Source: UniProtKB
Nitrobenzene metabolic process Source: BHF-UCL
Response to estrogen Source: UniProtKB
Xenobiotic catabolic process Source: BHF-UCL

Cellular Location

Cytoplasm

PTM

The N-terminus is blocked.

Chen, T., Jinlin, D., Wang, F., Yuan, Z., Xue, J., Lu, T., ... & Zhang, Y. (2022). GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling. Cancer Letters, 538, 215692.

Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., ... & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 741.

Juang, J. M. J., Binda, A., Lee, S. J., Hwang, J. J., Chen, W. J., Liu, Y. B., ... & Antzelevitch, C. (2020). GSTM3 variant is a novel genetic modifier in Brugada syndrome, a disease with risk of sudden cardiac death. EBioMedicine, 57, 102843.

Wang, S., Yang, J., You, L., Dai, M., & Zhao, Y. (2020). GSTM3 function and polymorphism in cancer: emerging but promising. Cancer management and research, 12, 10377.

Moghimi, M., Sobhan, M. R., Jarahzadeh, M. H., Morovati-Sharifabad, M., Aghili, K., Ahrar, H., ... & Neamatzadeh, H. (2019). Association of GSTM1, GSTT1, GSTM3, and GSTP1 genes polymorphisms with susceptibility to osteosarcoma: a case-control study and meta-analysis. Asian Pacific Journal of Cancer Prevention: APJCP, 20(3), 675.

Li, G., Cai, Y., Wang, C., Huang, M., & Chen, J. (2019). LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells. Journal of neuro-oncology, 143, 525-536.

Llavanera, M., Delgado-Bermúdez, A., Fernandez-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., ... & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of animal science and biotechnology, 10(1), 1-11.

Checa-Rojas, A., Delgadillo-Silva, L. F., del Castillo Velasco-Herrera, M., Andrade-Domínguez, A., Gil, J., Santillán, O., ... & Encarnación-Guevara, S. (2018). GSTM3 and GSTP1: novel players driving tumor progression in cervical cancer. Oncotarget, 9(31), 21696.

Wang, Y., Yang, Z. Y., Chen, Y. H., Li, F., Shen, H., Yu, Y., ... & Shen, Z. Y. (2018). A novel functional polymorphism of GSTM 3 reduces clear cell renal cell carcinoma risk through enhancing its expression by interfering miR‐556 binding. Journal of Cellular and Molecular Medicine, 22(6), 3005-3015.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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