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Mouse Anti-IFI35 Recombinant Antibody (1C9) (CBMAB-I0253-YY)

This product is Mouse antibody that recognizes IFI35. The antibody 1C9 can be used for immunoassay techniques such as: FC, IF, IHC, WB
See all IFI35 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C9
Antibody Isotype
IgG2a
Application
FC, IF, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Interferon Induced Protein 35
Introduction
IFI35 (Interferon Induced Protein 35) is a Protein Coding gene. Diseases associated with IFI35 include Stomatitis. Among its related pathways are Cytokine Signaling in Immune system and Innate Immune System. An important paralog of this gene is NMI.
Entrez Gene ID
3430
UniProt ID
P80217
Alternative Names
Interferon Induced Protein 35
Function
Acts as a signaling pathway regulator involved in innate immune system response (PubMed:26342464, PubMed:29038465, PubMed:29350881).

In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed:10779520, PubMed:10950963).

In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed:26342464).

In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881).

Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed:29038465).

Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed:29038465).
Biological Process
Innate immune response Source: UniProtKB-KW
Macrophage activation involved in immune response Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of inflammatory response Source: UniProtKB
Positive regulation of innate immune response Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm; Secreted; Nucleus. Cytoplasmic IFI35 localizes in punctate granular structures (PubMed:10950963). Nuclear localization increased is stimulated by IFN-alpha (PubMed:8288566, PubMed:10950963). Extracelullar following secretion by macrophage (PubMed:29038465).
PTM
Phosphorylated. Dephosphorylation correlates with the formation of a complex with NMI.

Gao, X., Zhang, Y., Zheng, J., Yang, X., Wang, Y., Qin, Q., ... & Huang, Y. (2022). Grouper interferon-induced protein 35, a CP-interacting protein, inhibits fish nodavirus replication via positively regulating host interferon and inflammatory immune response. Fish & Shellfish Immunology, 128, 113-122.

Jia, Y. Q., Wang, X. W., Chen, X., Qiu, X. X., Wang, X. L., & Yang, Z. Q. (2022). Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus. Journal of Veterinary Medical Science, 84(3), 473-483.

Chai, D., Shi, S. Y., Sobhani, N., Ding, J., Zhang, Z., Jiang, N., ... & Bai, J. (2022). IFI35 promotes renal cancer progression by inhibiting pSTAT1/pSTAT6-Dependent autophagy. Cancers, 14(12), 2861.

Zhou, P., Ma, L., Rao, Z., Li, Y., Zheng, H., He, Q., & Luo, R. (2021). Duck tembusu virus infection promotes the expression of duck interferon-induced protein 35 to counteract RIG-I antiviral signaling in duck embryo fibroblasts. Frontiers in Immunology, 12, 711517.

Yang, H., Winkler, W., & Wu, X. (2021). Interferon Inducer IFI35 Regulates RIG-I-Mediated Innate Antiviral Response through Mutual Antagonism with Influenza Virus Protein NS1. Journal of Virology, 95(11), 10-1128.

Hu, Y., Wang, B., Yi, K., Lei, Q., Wang, G., & Xu, X. (2021). IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells. Cancer Cell International, 21(1), 1-12.

Jian, D., Wang, W., Zhou, X., Jia, Z., Wang, J., Yang, M., ... & Zhu, J. (2018). Interferon‐induced protein 35 inhibits endothelial cell proliferation, migration and re‐endothelialization of injured arteries by inhibiting the nuclear factor‐kappa B pathway. Acta physiologica, 223(3), e13037.

Gounder, A. P., Yokoyama, C. C., Jarjour, N. N., Bricker, T. L., Edelson, B. T., & Boon, A. C. (2018). Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer. PLoS Pathogens, 14(4), e1007001.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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