Rabbit Anti-IMPA1 Recombinant Antibody (BA0148) (CBMAB-0374CQ)

Name: Rabbit Anti-IMPA1 Recombinant Antibody (BA0148)
SKU: CBMAB-0374CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

BA0148

Application

FC, WB, IP, IF

Immunogen

Human IMPA1 aa 50-150

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Inositol Monophosphatase 1

Introduction

The IMPA1 gene encodes inositol monophosphatase-1 (EC 3.1.3.25), an enzyme critical for recovery of the inositol cycle. It is important both for de novo synthesis of inositol and for the recycling of inositol polyphosphates generated upon receptor activation that relay signaling via intracellular second messengers. IMPA1 gene contains at least 9 exons and spans more than 20 kb. In the 3-prime untranslated part of the gene, they observed a G-to-A transition and also 2 short sequences similar to the inositol/cholin-responsive element consensus. They postulated that 2 additional IMPA-like transcripts originate from the human genome, 1 from a position close to IMPA1 itself on chromosome 8 and the other from 18. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13.

Entrez Gene ID

Human	3612
Mouse	55980
Rat	83523

UniProt ID

Human	P29218
Mouse	O55023
Rat	P97697

Alternative Names

IMP; IMPA

Function

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.

Biological Process

Inositol biosynthetic processIEA:UniProtKB-UniPathway
Inositol metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Inositol phosphate dephosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Phosphate-containing compound metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylinositol biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylinositol phosphate biosynthetic processIEA:InterPro
Signal transductionManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Mental retardation, autosomal recessive 59 (MRT59):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT59 transmission pattern is consistent with autosomal recessive inheritance.

Yang, S. Y., Xie, Y. F., Zhang, T. M., Deng, L., Liao, L., Hu, S. Y., ... & Li, D. Q. (2023). Inositol monophosphatase 1 (IMPA1) promotes triple‐negative breast cancer progression through regulating mTOR pathway and EMT process. Cancer Medicine, 12(2), 1602-1615.

Pessoa, A. L. S., Quesada, A. A., Nóbrega, P. R., Viana, A. P. O., de Oliveira, K. T., Figueiredo, T., ... & Kok, F. (2023). Neuropsychological Characterization of Autosomal Recessive Intellectual Developmental Disorder 59 Associated with IMPA1 (MRT59). Brain Sciences, 13(7), 1048.

Padinjat, R., Saha, S., & Krishnan, H. (2022). IMPA1 dependent regulation of plasma membrane phosphatidylinositol 4, 5-bisphosphate turnover and calcium signalling by lithium. bioRxiv, 2022-10.

Andreassi, C., Luisier, R., Crerar, H., Darsinou, M., Blokzijl-Franke, S., Lenn, T., ... & Riccio, A. (2021). Cytoplasmic cleavage of IMPA1 3′ UTR is necessary for maintaining axon integrity. Cell Reports, 34(8).

Figueiredo, T., Mendes, A. P., Moreira, D. P., Goulart, E., Oliveira, D., Kobayashi, G. S., ... & Zatz, M. (2021). Inositol monophosphatase 1 (IMPA1) mutation in intellectual disability patients impairs neurogenesis but not gliogenesis. Molecular Psychiatry, 26(7), 3558-3571.

Pillai, R. A., Islam, M. O., Selvam, P., Sharma, N., Chu, A. H., Watkins, O. C., ... & Chan, S. Y. (2021). Placental inositol reduced in gestational diabetes as glucose alters inositol transporters and IMPA1 enzyme expression. The Journal of Clinical Endocrinology & Metabolism, 106(2), e875-e890.

Bogatikov, E., Lindblad, I., Punga, T., & Punga, A. R. (2020). miR-1933-3p is upregulated in skeletal muscles of MuSK+ EAMG mice and affects Impa1 and Mrpl27. Neuroscience research, 151, 46-52.

Walker, C. P., Pessoa, A. L., Figueiredo, T., Rafferty, M., Melo, U. S., Nóbrega, P. R., ... & Cho, R. Y. (2019). Loss-of-function mutation in inositol monophosphatase 1 (IMPA1) results in abnormal synchrony in resting-state EEG. Orphanet Journal of Rare Diseases, 14(1), 1-10.

Rosette, C., & Murray PhD, M. (2018). P-29 Preparing to Test the Effects of Omega-3 Fatty Acids on Inositol Levels and ISYNA & IMPA1 Gene Expression in Mammalian Cells.

de Farias, A. A., Nunes, K., Lemes, R. B., Moura, R., Fernandes, G. R., Melo, U. S., ... & Santos, S. (2018). Origin and age of the causative mutations in KLC2, IMPA1, MED25 and WNT7A unravelled through Brazilian admixed populations. Scientific reports, 8(1), 16552.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-IMPA1 Recombinant Antibody (BA0149) (CAT#: CBMAB-0373CQ)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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