Rabbit Anti-INPP5D Recombinant Antibody (
9G11) (V2LY-0725-LY972)

Name: Rabbit Anti-INPP5D Recombinant Antibody (9G11)
SKU: V2LY-0725-LY972
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-INPP5D Recombinant Antibody (9G11) (V2LY-0725-LY972) in WB

Western Blot
Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate
All lanes: INPP5D antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 134, 110 kDa
Observed band size: 145 kDa

Rabbit Anti-INPP5D Recombinant Antibody (9G11) (V2LY-0725-LY972) in IHC

IHC image of V2LY-0725-LY972 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-INPP5D Recombinant Antibody (9G11) (V2LY-0725-LY972) in IP

Immunoprecipitating SHIP in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY972 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY972(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9G11

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human SHIP.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Inositol Polyphosphate-5-Phosphatase D

Entrez Gene ID

3635

UniProt ID

Q92835

Function

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:8723348, PubMed:10764818, PubMed:8769125).
Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9108392, PubMed:10764818, PubMed:8769125).
Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed:16682172).
Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.

Biological Process

Apoptotic processIEA:UniProtKB-KW
Determination of adult lifespanIEA:Ensembl
Immunoglobulin mediated immune responseIEA:Ensembl
Intracellular signal transductionIEA:Ensembl
Negative regulation of B cell proliferationIEA:Ensembl
Negative regulation of bone resorptionIEA:Ensembl
Negative regulation of immune responseIEA:Ensembl
Negative regulation of interleukin-6 productionIEA:Ensembl
Negative regulation of monocyte differentiationIEA:Ensembl
Negative regulation of neutrophil differentiationIEA:Ensembl
Negative regulation of osteoclast differentiationIEA:Ensembl
Negative regulation of signal transductionIEA:Ensembl
Phosphate-containing compound metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Phosphatidylinositol biosynthetic processTAS:Reactome
Phosphatidylinositol dephosphorylationIEA:InterPro
Positive regulation of apoptotic processIEA:Ensembl
Positive regulation of B cell differentiationIEA:Ensembl
Positive regulation of erythrocyte differentiationIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
T cell receptor signaling pathwayTAS:Reactome

Cellular Location

Cytoplasm; Cell membrane; Membrane raft; Cytoplasm, cytoskeleton; Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization.

PTM

Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

Castranio, E. L., Hasel, P., Haure‐Mirande, J. V., Ramirez Jimenez, A. V., Hamilton, B. W., Kim, R. D., ... & Ehrlich, M. E. (2023). Microglial INPP5D limits plaque formation and glial reactivity in the PSAPP mouse model of Alzheimer's disease. Alzheimer's & Dementia, 19(6), 2239-2252.

Olufunmilayo, E. O., & Holsinger, R. D. (2023). INPP5D/SHIP1: Expression, Regulation and Roles in Alzheimer’s Disease Pathophysiology. Genes, 14(10), 1845.

Lin, P. B. C., Tsai, A. P. Y., Soni, D., Lee‐Gosselin, A., Moutinho, M., Puntambekar, S. S., ... & Oblak, A. L. (2023). INPP5D deficiency attenuates amyloid pathology in a mouse model of Alzheimer's disease. Alzheimer's & Dementia, 19(6), 2528-2537.

Luan, W., Sun, Z., Wu, C., Tao, M., & Shen, X. (2023). Neuroinflammation after ischemic stroke involves INPP5D expression mediated by the TMPO-AS1-PU. 1 complex. Neurological Research, 45(4), 319-333.

Gandy, S., & Ehrlich, M. E. (2023). miR155, TREM2, INPP5D: Disease stage and cell type are essential considerations when targeting clinical interventions based on mouse models of Alzheimer’s amyloidopathy. Journal of Neuroinflammation, 20(1), 1-5.

Zajac, D. J., Simpson, J., Zhang, E., Parikh, I., & Estus, S. (2023). Expression of INPP5D Isoforms in Human Brain: Impact of Alzheimer’s Disease Neuropathology and Genetics. Genes, 14(3), 763.

Iguchi, A., Takatori, S., Kimura, S., Muneto, H., Wang, K., Etani, H., ... & Tomita, T. (2023). INPP5D modulates TREM2 loss-of-function phenotypes in a β-amyloidosis mouse model. Iscience, 26(4).

Tsai, A. P., Lin, P. B. C., Dong, C., Moutinho, M., Casali, B. T., Liu, Y., ... & Nho, K. (2021). INPP5D expression is associated with risk for Alzheimer's disease and induced by plaque-associated microglia. Neurobiology of disease, 153, 105303.

Yao, X., Risacher, S. L., Nho, K., Saykin, A. J., Wang, Z., Shen, L., & Alzheimer's Disease Neuroimaging Initiative. (2019). Targeted genetic analysis of cerebral blood flow imaging phenotypes implicates the INPP5D gene. Neurobiology of aging, 81, 213-221.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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