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Rabbit Anti-KPNB1 Recombinant Antibody (E1F1G) (CBMAB-CP1311-LY)

The product is antibody recognizes Importin β1. The antibody E1F1G immunoassay techniques such as: WB,IP.
See all KPNB1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
E1F1G
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn486 of human importin β1 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
karyopherin (importin) beta 1
Entrez Gene ID
Human3837
Mouse16211
Rat24917
UniProt ID
HumanQ14974
MouseP70168
RatP52296
Function
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.
Biological Process
Astral microtubule organizationManual Assertion Based On ExperimentIMP:UniProtKB
Establishment of mitotic spindle localizationManual Assertion Based On ExperimentIMP:UniProtKB
Establishment of protein localizationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic chromosome movement towards spindle poleManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
NLS-bearing protein import into nucleusManual Assertion Based On ExperimentTAS:ProtInc
Protein import into nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Ran protein signal transductionManual Assertion Based On ExperimentIMP:GO_CentralRibosomal protein import into nucleusManual Assertion Based On ExperimentIDA:UniProtKB
RNA import into nucleusManual Assertion Based On ExperimentIDA:ComplexPortal
Cellular Location
Cytoplasm
Nucleus envelope
PTM
Mono-ADP-ribosylated by PARP16.

Shi, Q., Lin, M., Cheng, X., Zhang, Z., Deng, S., Lang, K., ... & Sun, X. (2023). KPNB1-mediated nuclear import in cancer. European Journal of Pharmacology, 175925.

Ye, Z., Yang, Y., Wei, Y., Li, L., Wang, X., & Zhang, J. (2022). PCDH1 promotes progression of pancreatic ductal adenocarcinoma via activation of NF-κB signalling by interacting with KPNB1. Cell Death & Disease, 13(7), 633.

Du, W., Zhu, J., Zeng, Y., Liu, T., Zhang, Y., Cai, T., ... & Huang, J. A. (2021). KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway. Cell Death & Differentiation, 28(4), 1284-1300.

Cao, Y., Wang, F., Chen, Y., Wang, Y., Song, H., & Long, J. (2021). CircPITX1 regulates proliferation, angiogenesis, migration, invasion, and cell cycle of human glioblastoma cells by targeting miR-584-5p/KPNB1 Axis. Journal of Molecular Neuroscience, 71, 1683-1695.

Zhu, Z. C., Liu, J. W., Yang, C., Li, M. J., Wu, R. J., & Xiong, Z. Q. (2019). Targeting KPNB1 overcomes TRAIL resistance by regulating DR5, Mcl-1 and FLIP in glioblastoma cells. Cell death & disease, 10(2), 118.

Wang, T., Huang, Z., Huang, N., Peng, Y., Gao, M., Wang, X., & Feng, W. (2019). Inhibition of KPNB1 inhibits proliferation and promotes apoptosis of chronic myeloid leukemia cells through regulation of E2F1. OncoTargets and therapy, 12, 10455.

Park, C. H., Ham, S. W., Shin, H., & Oh, K. S. (2019). Inhibition of kPNB1 and NUPR1 binding increase the anti-cancer drug sensitivity. Cancer Research, 79(13_Supplement), 5138-5138.

Zhu, Z. C., Liu, J. W., Li, K., Zheng, J., & Xiong, Z. Q. (2018). KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. Oncogene, 37(22), 2936-2952.

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For research use only. Not intended for any clinical use.

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