Mouse Anti-MYO9B (AA 201-290) Recombinant Antibody (CBFYM-2995) (CBMAB-M3190-FY)

Name: Mouse Anti-MYO9B (AA 201-290) Recombinant Antibody (CBFYM-2995)
SKU: CBMAB-M3190-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2995

Application

ELISA

Immunogen

Partial recombinant corresponding to aa201-290 from human MYO9B (AAH18108) with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human

Antibody Isotype

IgG2a, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 201-290

More Infomation

Target

Full Name

MYOSIN IXB

Introduction

This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9. The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4650

UniProt ID

Q13459

Alternative Names

Myosin IXB; Unconventional Myosin-9b; MYR5; Unconventional Myosin-IXb; Myosin-IXb; CELIAC4

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed:9490638).

Also acts as a GTPase activator for RHOA (PubMed:9490638, PubMed:26529257).

Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257).

Biological Process

Actin filament-based movement Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: UniProtKB
Roundabout signaling pathway Source: UniProtKB

Cellular Location

Cytoskeleton
Other locations
cell cortex
perinuclear region
Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.

Involvement in disease

Celiac disease 4 (CELIAC4):
A multifactorial, chronic disorder of the small intestine caused by intolerance to gluten. It is characterized by immune-mediated enteropathy associated with failed intestinal absorption, and malnutrition. In predisposed individuals, the ingestion of gluten-containing food such as wheat and rye induces a flat jejunal mucosa with infiltration of lymphocytes.

Zhang, J., Zou, Y., Chen, L., Sun, F., Xu, Q., Zhou, Q., ... & Wang, C. Y. (2023). Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset. Nature Communications, 14(1), 5977.

Lee, B. S., Murray, C., Liu, J., Kim, M., Hwang, M. S., Yueh, T., ... & Kim, D. G. (2023). The myosin and RhoGAP MYO9B influences osteocyte dendrite growth and responses to mechanical stimuli. Frontiers in Bioengineering and Biotechnology, 11.

Cipriani, S., Guerrero‐Valero, M., Tozza, S., Zhao, E., Vollmer, V., Beijer, D., ... & Bolino, A. (2023). Mutations in MYO9B are associated with Charcot–Marie–Tooth disease type 2 neuropathies and isolated optic atrophy. European Journal of Neurology, 30(2), 511.

Hemkemeyer, S. A., Liu, Z., Vollmer, V., Xu, Y., Lohmann, B., & Bähler, M. (2022). The RhoGAP‐myosin Myo9b regulates ocular lens pit morphogenesis. Developmental Dynamics, 251(11), 1897-1907.

Hemkemeyer, S. A., Vollmer, V., Schwarz, V., Lohmann, B., Honnert, U., Taha, M., ... & Bähler, M. (2021). Local Myo9b RhoGAP activity regulates cell motility. Journal of Biological Chemistry, 296.

Comer, S., Nagy, Z., Bolado, A., von Kriegsheim, A., Gambaryan, S., Walter, U., ... & Smolenski, A. (2020). The RhoA regulators Myo9b and GEF‐H1 are targets of cyclic nucleotide‐dependent kinases in platelets. Journal of Thrombosis and Haemostasis, 18(11), 3002-3012.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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