Mouse Anti-NCAPG Recombinant Antibody (2C5) (CBMAB-N1371-WJ)

Name: Mouse Anti-NCAPG Recombinant Antibody (2C5)
SKU: CBMAB-N1371-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C5

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

non-SMC condensin I complex, subunit G

Introduction

This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Entrez Gene ID

64151

UniProt ID

Q9BPX3

Alternative Names

Non-SMC Condensin I Complex Subunit G; Chromosome Condensation Protein G; Chromosome-Associated Protein G; Melanoma Antigen NY-MEL-3; Condensin Subunit CAP-G; XCAP-G Homolog; CAPG; Non-SMC Condensin I Complex, Subunit G;

Function

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Biological Process

Cell division Source: UniProtKB-KW
Mitotic chromosome condensation Source: UniProtKB
Positive regulation of chromosome condensation Source: ComplexPortal
Positive regulation of chromosome segregation Source: Ensembl
Positive regulation of chromosome separation Source: Ensembl

Cellular Location

Nucleus
Cytoplasm
Other locations
Chromosome
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

PTM

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPH subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

Zhang, R., Ai, J., Wang, J., Sun, C., Lu, H., He, A., ... & Liao, W. (2022). NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN. Journal of translational medicine, 20(1), 325.

Sun, H., Zhang, H., Yan, Y., Li, Y., Che, G., Zhou, C., ... & Ma, H. (2022). NCAPG promotes the oncogenesis and progression of non-small cell lung cancer cells through upregulating LGALS1 expression. Molecular cancer, 21(1), 55.

Shi, Y., Ge, C., Fang, D., Wei, W., Li, L., Wei, Q., & Yu, H. (2022). NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial–mesenchymal transition by activating the Wnt/β-catenin signaling pathway. Cancer Cell International, 22(1), 1-13.

Cai, X., Gao, J., Shi, C., zhi Guo, W., Guo, D., & Zhang, S. (2022). The role of NCAPG in various of tumors. Biomedicine & Pharmacotherapy, 155, 113635.

Yuan, Y., Jiang, X., Tang, L., Wang, J., Zhang, D., Cho, W. C., & Duan, L. (2022). FOXM1/lncRNA TYMSOS/miR-214-3p–mediated high expression of NCAPG correlates with poor prognosis and cell proliferation in non–small cell lung carcinoma. Frontiers in Molecular Biosciences, 8, 785767.

Wu, Y., Lin, Y., Pan, J., Tu, X., Xu, Y., Li, H., & Chen, Y. (2021). NCAPG promotes the progression of lung adenocarcinoma via the TGF-β signaling pathway. Cancer Cell International, 21(1), 443.

Sun, D. P., Lin, C. C., Hung, S. T., Kuang, Y. Y., Hseu, Y. C., Fang, C. L., & Lin, K. Y. (2020). Aberrant expression of NCAPG is associated with prognosis and progression of gastric cancer. Cancer management and research, 7837-7846.

Jiang, L., Ren, L., Chen, H., Pan, J., Zhang, Z., Kuang, X., ... & Song, L. (2020). NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer. Cell death & disease, 11(7), 547.

Wang, Y., Gao, B., Tan, P. Y., Handoko, Y. A., Sekar, K., Deivasigamani, A., ... & Hui, K. M. (2019). Genome-wide CRISPR knockout screens identify NCAPG as an essential oncogene for hepatocellular carcinoma tumor growth. The FASEB Journal, 33(8), 8759.

Gong, C., Ai, J., Fan, Y., Gao, J., Liu, W., Feng, Q., ... & Wu, L. (2019). NCAPG promotes the proliferation of hepatocellular carcinoma through PI3K/AKT signaling. OncoTargets and therapy, 12, 8537.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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