Mouse Anti-NEK2 Recombinant Antibody (2A10) (CBMAB-N1924-WJ)

Name: Mouse Anti-NEK2 Recombinant Antibody (2A10)
SKU: CBMAB-N1924-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A10

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NIMA (never in mitosis gene a)-related kinase 2

Introduction

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Entrez Gene ID

4751

UniProt ID

P51955

Alternative Names

NIMA Related Kinase 2; NIMA (Never In Mitosis Gene A)-Related Kinase 2; Protein Phosphatase 1, Regulatory Subunit 111; NimA-Related Protein Kinase 2; NimA-Like Protein Kinase 1; EC 2.7.11.1; HsPK 21; NEK2A;

Function

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434).

NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143).

Involved in the regulation of centrosome disjunction (PubMed:26220856).

Isoform 1:
Phosphorylates and activates NEK11 in G1/S-arrested cells.

Isoform 2:
Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

Biological Process

Blastocyst development Source: Ensembl
Cell division Source: UniProtKB-KW
Centrosome separation Source: UniProtKB
Chromosome segregation Source: UniProtKB
Meiotic cell cycle Source: UniProtKB-KW
Mitotic cell cycle Source: ProtInc
Mitotic sister chromatid segregation Source: Ensembl
Mitotic spindle assembly Source: Ensembl
Negative regulation of centriole-centriole cohesion Source: UniProtKB
Negative regulation of DNA binding Source: Ensembl
Positive regulation of telomerase activity Source: BHF-UCL
Positive regulation of telomere capping Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
Regulation of mitotic centrosome separation Source: UniProtKB
Regulation of mitotic nuclear division Source: ProtInc
Spindle assembly Source: UniProtKB

Cellular Location

Isoform 1:
Nucleus
nucleolus
Cytoplasm
Cytoskeleton
centrosome
spindle pole
Other locations
kinetochore
centromere
Note: STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.
Isoform 2:
Cytoplasm
Note: Predominantly cytoplasmic.
Isoform 4:
Nucleus
Cytoskeleton
centrosome
Note: Predominantly nuclear.

Involvement in disease

Retinitis pigmentosa 67 (RP67):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

PTM

Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.

Shah, D., Joshi, M., & Patel, B. M. (2022). Role of NIMA‐related kinase 2 in lung cancer: Mechanisms and therapeutic prospects. Fundamental & Clinical Pharmacology, 36(5), 766-776.

Mori, T., Onodera, Y., Itokazu, M., Takehara, T., Shigi, K., Iwawaki, N., ... & Teramura, T. (2022). Depletion of NIMA-related kinase Nek2 induces aberrant self-renewal and apoptosis in stem/progenitor cells of aged muscular tissues. Mechanisms of Ageing and Development, 201, 111619.

Gu, X., Wang, Z., & Pan, Q. (2022). Overexpression of NIMA related kinase 2 in multiple myeloma and its relevance with disease features and prognosis to bortezomib treatment. Journal of Clinical Pharmacy and Therapeutics, 47(10), 1690-1697.

Dana, D., Das, T., Choi, A., Bhuiyan, A. I., Das, T. K., Talele, T. T., & Pathak, S. K. (2022). Nek2 kinase signaling in malaria, bone, immune and kidney disorders to metastatic cancers and drug resistance: progress on Nek2 inhibitor development. Molecules, 27(2), 347.

Wang, X., Chen, K., Liu, H., Huang, Z., Chen, X., & Yin, L. (2019). Prognostic significance of NEK2 in human solid tumors: a systematic review and meta-analysis. Bioscience Reports, 39(1), BSR20180618.

Yao, Y., Su, J., Zhao, L., Luo, N., Long, L., & Zhu, X. (2019). NIMA-related kinase 2 overexpression is associated with poor survival in cancer patients: a systematic review and meta-analysis. Cancer Management and Research, 455-465.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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