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Mouse Anti-RHOH Recombinant Antibody (CBYJT-1630) (CBMAB-T0676-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to RHOH (Ras Homolog Family Member H). The antibody can be used for immunoassay techniques, such as ELISA.
See all RHOH antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1630
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ras homolog gene family, member H
Introduction
RHOH is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. RHOH is expressed in hematopoietic cells, where it plays a role as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts.
Entrez Gene ID
399
UniProt ID
Q15669
Alternative Names
Ras Homolog Family Member H; Ras Homolog Gene Family, Member H; GTP-Binding Protein TTF; ARHH; TTF; Rho-Related GTP-Binding Protein RhoH; Translocation Three Four Protein; TTF, Translocation Three Four
Function
Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells (By similarity).
Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.
Biological Process
Biological Process actin filament organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process cell projection assemblyManual Assertion Based On ExperimentIBA:GO_Central
Biological Process cortical cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process engulfment of apoptotic cellManual Assertion Based On ExperimentIBA:GO_Central
Biological Process establishment or maintenance of cell polarityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mast cell activationIEA:Ensembl
Biological Process motor neuron axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of T cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Rac protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of cell shapeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of neutrophil migrationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of transcription, DNA-templated1 PublicationNAS:UniProtKB
Biological Process T cell differentiation1 PublicationNAS:UniProtKB
Cellular Location
Cytoplasm
Cell membrane
Colocalizes together with ZAP70 in the immunological synapse.
Involvement in disease
Epidermodysplasia verruciformis 4 (EV4):
A form of epidermodysplasia verruciformis, a rare genodermatosis associated with a high risk of skin carcinoma that results from an abnormal susceptibility to infection by specific human papillomaviruses, including the oncogenic HPV5. Infection leads to the early development of disseminated flat wart-like and pityriasis versicolor-like skin lesions. Cutaneous Bowen's carcinomas in situ and invasive squamous cell carcinomas develop in about half of the patients, mainly on sun-exposed skin areas. EV4 patients have decreased number of naive T cells, increased memory and effector T cells, and impaired T-cell receptor signaling. EV4 inheritance is autosomal recessive.
PTM
Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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