Mouse Anti-S. pyogenes HS SLO Recombinant Antibody (CBMW-H1142) (CBMAB-V208-1302-FY)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
CBMW-H1142
Application
WB, ELISA
Immunogen
Recombinant protein-Hemolytic Streptococcus SLO
Specificity
S. pyogenes
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Streptococcus Pyogenes Hs Slo
Introduction
Streptococcus pyogenes is a species of Gram-positive bacterium in the genus Streptococcus.
Alternative Names
S. pyogenes HS SLO; Streptococcus pyogenes HS SLO
Function
A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Biological Process
Hemolysis in another organism
Cellular Location
Secreted
Host cell membrane
Probably secreted as soluble protein by the accessory Sec system (By similarity).
It then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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