Mouse Anti-SPAG5 Recombinant Antibody (CBFYM-3139) (CBMAB-M3336-FY)
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Basic Information
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074).
In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792).
Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806).
In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116).
May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).
Biological Process chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process establishment of spindle orientationIEA:Ensembl
Biological Process mitotic sister chromatid segregationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of intracellular transportManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein localization to centrosomeManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of attachment of spindle microtubules to kinetochoreManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process spindle organizationManual Assertion Based On ExperimentIMP:UniProtKB
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, spindle pole
Chromosome, centromere, kinetochore
Midbody
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasmic granule
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite
Colocalizes with PCM1 at centriolar satellites throughout the cell cycle (PubMed:26297806).
In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase (PubMed:27462074).
Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity).
In non-mitotic non-stressed cells, shows a microtubuli pattern. In arsenite-stressed cells, accumulates in stress granules
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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