Antibody array, also known as antibody microarray, is a high-throughput technique that is able to detect multiple proteins simultaneously with minimal sample volume requirements. Compared with other traditional, single analyte methods of protein analysis, antibody array has demonstrated a number of advantages such as small sample volume and antibody concentration requirements, higher format versatility, reproducibility. These advantages support the increasing use of antibody arrays in clinical applications including disease marker discovery for diagnosis, prognosis, and drug response, characterization of signaling and protein pathways, and modifications associated with disease development and progression. Now, antibody arrays have become more standardized and user-friendly experimental procedures.
Fig.2 Designing and developing antibody arrays (Wingren et al)
Demonstration of glycan and protein detection with antibody arrays. Antibody arrays were incubated with no serum, unlabeled serum or digoxigenin-labeled serum. The arrays were then incubated with biotinylated SNA lectin, followed by detection with Cy5-labeled antidigoxigenin (red, 633 nm) and Cy3-labeled antibiotin (green, 543 nm). Fluorescence from the spots is shown in both color channels from the indicated antibodies.
Haab B. B. (2006). Applications of antibody array platforms. Curr Opin Biotechnol, 17(4): 415-421.
Antibody array has emerged as an important tool within proteomics, providing unique opportunities for multiplexed protein expression profiling in research, health and disease. In this context, Creative Biolabs has developed a recombinant antibody array technology platform for protein profiling of crude, directly-labelled proteomes. Various antibody array products are provided, such as Cytokine Antibody Array, Angiogenesis Antibody Array and Cancer Biomarker Antibody Array. Also, Creative Biolabs provides customization services for your exact application needs.