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Antibody Array

Antibody array, also known as antibody microarray, is a high-throughput technique that is able to detect multiple proteins simultaneously with minimal sample volume requirements. Compared with other traditional, single analyte methods of protein analysis, antibody array has demonstrated a number of advantages such as small sample volume and antibody concentration requirements, higher format versatility, reproducibility. These advantages support the increasing use of antibody arrays in clinical applications including disease marker discovery for diagnosis, prognosis, and drug response, characterization of signaling and protein pathways, and modifications associated with disease development and progression. Now, antibody arrays have become more standardized and user-friendly experimental procedures.

Fig.2 Designing and developing antibody arrays (Wingren et al)

Phospho-kinase antibody array

Representative image of antibody arrays showing UV‐induced upregulation of the phosphorylated form of p38ɑ (T180/Y182) at the indicated time points.

Lenain, C., Gamboa, B., Perrin, A., Séraïdaris, A., Bertino, B., Rival, Y., ... & Méhul, B. (2018). Monitoring UV‐induced signalling pathways in an ex vivo skin organ culture model using phospho-antibody array. Experimental dermatology,27(5), 470-472.

Phospho-kinase antibody array

Representative antibody arrays. Antibody array chips encompass 507 cytokines in duplicates probed with whole lysates from paired gastric cancer (GC) and non-GC mucosa in patients #3 (A), #5 (B), #6 (C) and #7 (D) (a1, b1, c1 and d1: adjacent noncancerous tissue; a2, b2, c2 and d2: gastric carcinoma tissue).

Quan, X., Ding, Y., Feng, R., Zhu, X., & Zhang, Q. (2017). Expression profile of cytokines in gastric cancer patients using proteomic antibody microarray. Oncology letters, 14(6), 7360-7366.

Evaluation of microarray quality in terms of array design and printing markers. (A) Representative image of a microarray slide with 14 identical subarrays. Each subarray (n = 14) is divided into 3 segments separated by dispensed rows of reference marker replicate spots (n = 4). (B) Blank replicate spots (n = 5) detected in the marked area. No signal intensity was obtained from the dispensed blank replicate spots after scanning the microarray using a wavelength of 633 nm, while positive spots at 543 nm (Cadaverine) confirmed that spotting had occurred.

Delfani, P., Mellby, L. D., Nordström, M., Holmér, A., Ohlsson, M., Borrebaeck, C. A., & Wingren, C. (2016). Technical advances of the recombinant antibody microarray technology platform for clinical immunoproteomics. PLoS One, 11(7), e0159138.

Demonstration of glycan and protein detection with antibody arrays. Antibody arrays were incubated with no serum, unlabeled serum or digoxigenin-labeled serum. The arrays were then incubated with biotinylated SNA lectin, followed by detection with Cy5-labeled antidigoxigenin (red, 633 nm) and Cy3-labeled antibiotin (green, 543 nm). Fluorescence from the spots is shown in both color channels from the indicated antibodies.

Haab B. B. (2006). Applications of antibody array platforms. Curr Opin Biotechnol, 17(4): 415-421.

Antibody array has emerged as an important tool within proteomics, providing unique opportunities for multiplexed protein expression profiling in research, health and disease. In this context, Creative Biolabs has developed a recombinant antibody array technology platform for protein profiling of crude, directly-labelled proteomes. Various antibody array products are provided, such as Cytokine Antibody Array, Angiogenesis Antibody Array and Cancer Biomarker Antibody Array. Also, Creative Biolabs provides customization services for your exact application needs.