Human Recombinant ACP3 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY6342)

Name: Human Recombinant ACP3 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY6342
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant ACP3 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-382 and predicts a molecular mass of 43.9 kDa.

Molecule Mass

43.9 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-382

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC. ≥90% as determined by SEC-MALS(Routinely tested).

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

acid phosphatase, prostate

Function

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).
PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.

Biological Process

Adenosine metabolic process
Dephosphorylation
Lysosome organization
Neutrophil degranulation
Nucleotide metabolic process
Positive regulation of adenosine receptor signaling pathway
Purine nucleobase metabolic process
Regulation of sensory perception of pain
Thiamine metabolic process

Cellular Location

Isoform 1: Secreted
Isoform 2: Nucleus; Lysosome membrane; Cell membrane; Cytosol.
Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles. Colocalizes with LAMP2 on the lysosome membrane

PTM

N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection).

de Pizzol Júnior, J. P., Sasso-Cerri, E., & Cerri, P. S. (2018). Matrix metalloproteinase-1 and acid phosphatase in the degradation of the lamina propria of eruptive pathway of rat molars. Cells, 7(11), 206.

Jahnke, G., Nagy, Z. A., Koltai, G., Hajdu, E., & Májer, J. (2017). Absence of an acid phosphatase isozyme locus as a marker candidate for true to typeness in woodland grape (Vitis vinifera L. ssp. sylvestris Gmelin). Oeno One, 51(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ACP3 protein, His Tag (CAT#: V2LY-0526-LY6343)

Human Recombinant ACP3 protein, His Tag-1 (CAT#: V2LY-0526-LY6344)

Related Products

Human Recombinant ACP3 protein, His Tag (CAT#: V2LY-0526-LY6343)

Human Recombinant ACP3 protein, His Tag-1 (CAT#: V2LY-0526-LY6344)

Mouse Anti-ACP3 Recombinant Antibody (V2-179370) (CAT#: CBMAB-A0672-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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