Human Recombinant PRKAG1 protein, GST Tag (V2LY-0526-LY6298)

Name: Human Recombinant PRKAG1 protein, GST Tag
SKU: V2LY-0526-LY6298
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant PRKAG1 protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 68 kDa.

Molecule Mass

68 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 1

Function

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Biological Process

Cellular response to glucose starvationManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to nutrient levelsManual Assertion Based On ExperimentIDA:ComplexPortal
Fatty acid biosynthetic processIEA:UniProtKB-KW
Import into nucleusIEA:Ensembl
Positive regulation of gene expressionManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein kinase activityManual Assertion Based On ExperimentTAS:BHF-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of catalytic activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of glycolytic processManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of protein serine/threonine kinase activityIEA:InterPro
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
SpermatogenesisManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

cytoplasm
cytosol
membrane
nucleoplasm
nucleotide-activated protein kinase complex
nucleus

PTM

Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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