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Mouse Anti-PRMT4/CARM1 Recombinant Antibody (2B9-H11-H10) (CBMAB-Z0434-LY)

The product is antibody recognizes PRMT4/CARM1. The antibody 2B9-H11-H10 immunoassay techniques such as: WB, IP.
See all PRMT4/CARM1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2B9-H11-H10
Antibody Isotype
IgG1
Application
WB, IP

Basic Information

Immunogen
Purified recombinant human PRMT4/CARM1 protein fragments expressed in E.coli.
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 0.1 mg/ml BSA and 50% glycerol.
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Coactivator Associated Arginine Methyltransferase 1
Entrez Gene ID
Human10498
Mouse59035
UniProt ID
HumanQ86X55
MouseQ9WVG6
Alternative Names
PRMT4
Function
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
Biological Process
Aging Source: Ensembl
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Histone H3-R17 methylation Source: UniProtKB
Histone H3-R2 methylation Source: UniProtKB
Histone methylation Source: UniProtKB
Negative regulation of dendrite development Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of fat cell differentiation Source: UniProtKB
Regulation of intracellular estrogen receptor signaling pathway Source: UniProtKB
Regulation of lipid metabolic process Source: Reactome
Regulation of mRNA binding Source: Ensembl
Regulation of transcription, DNA-templated Source: UniProtKB
Response to cAMP Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Nucleus. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
PTM
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity).
Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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