Rabbit Anti-5-caC Recombinant Antibody (V2-623778) (CBMAB-MA265-YC)

Name: Rabbit Anti-5-caC Recombinant Antibody (V2-623778)
SKU: CBMAB-MA265-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-5-caC Recombinant Antibody (MA001YC) in Dot blot

Dot blot analysis of genomic 5fC, and 5caC in WT and PROSER1 KO HEK293 cells. 5fC, and 5caC are decreased in PROSER1 KO versus WT cells. Methylene Blue staining was performed to ensure that equal amounts of DNA were used from WT and PROSER1 KO cells. ...View More

Wang, X., Rosikiewicz, W., Sedkov, Y., Martinez, T., Hansen, B. S., Schreiner, P., ... & Herz, H. M. (2022). PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands. Life Science Alliance, 5(1).

Rabbit Anti-5-caC Recombinant Antibody (MA001YC) in Dot blot

5caC dot blot in WT and Tdg−/− mESC genomic DNA. Replicates as in (B) with methylene blue staining as loading control. Relative 5caC abundance when compared with WT control is determined through methylene blue normalization. ...View More

Nanan, K. K., Sturgill, D. M., Prigge, M. F., Thenoz, M., Dillman, A. A., Mandler, M. D., & Oberdoerffer, S. (2019). TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide. Iscience, 19, 326-339.

Datasheet

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Basic Information

Host Animal

Rabbit

Clone

V2-623778

Application

Nucleotide Array, ELISA, MeDIP

Immunogen

5-carboxylcytidine conjugated to keyhole limpet haemocyan (KLH)

Host Species

Rabbit

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES pH 7.5, 0.01% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

More Infomation

Target

Full Name

5-carboxylcytosine

Introduction

5-carboxylcytosine (5caC) is the final oxidized derivative of 5-methylcytosine (5mC). 5mC is oxidized to 5-hydroxymethylcytosine (5hmC) which is then oxidized to 5-formylcytosine (5fC) then 5caC. Each of these oxidation steps are catalyzed by the Ten-Eleven Translocation (TET) family of enzymes. 5fC can then be further oxidized to 5-carboxylcytosine (5caC) by TET. Both 5fC and 5caC can be converted to unmodified cytosine by Terminal deoxynucleotidyl transferase (TdT) or Thymine DNA glycosylase (TDG) by base excision repair.

Alternative Names

5-carboxylcytosine

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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