Rat Recombinant EFNB1 protein, His Tag (V2LY-0526-LY9367)

Name: Rat Recombinant EFNB1 protein, His Tag
SKU: V2LY-0526-LY9367
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant EFNB1 protein, His Tag consist of Amino Acid: 1-229 and predicts a molecular mass of 23.8 kDa.

Molecule Mass

23.8 kDa

Sequence

Amino Acid: 1-229

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ephrin B1

Research Area

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed:8070404, PubMed:7973638).

Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed:8070404, PubMed:7973638).

Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed:8070404, PubMed:7973638).

Can also bind EPHB2 and EPHB3 (PubMed:8070404).

Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity).

May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

Biological Process

Axon guidance Source: GO_Central
Cell adhesion Source: ProtInc
Cell-cell signaling Source: ProtInc
Embryonic pattern specification Source: Ensembl
Ephrin receptor signaling pathway Source: GO_Central
Neural crest cell migration Source: Ensembl
Positive regulation of T cell proliferation Source: Ensembl
T cell costimulation Source: Ensembl

Cellular Location

Cell membrane; Membrane raft. May recruit GRIP1 and GRIP2 to membrane raft domains.
Ephrin-B1 C-terminal fragment: Cell membrane
Ephrin-B1 intracellular domain: Nucleus. Colocalizes with ZHX2 in the nucleus.

Involvement in disease

Craniofrontonasal syndrome (CFNS):
X-linked inherited syndrome characterized by hypertelorism, coronal synostosis with brachycephaly, downslanting palpebral fissures, clefting of the nasal tip, joint anomalies, longitudinally grooved fingernails and other digital anomalies.

Topology

Extracellular: 28-237
Helical: 238-258
Cytoplasmic: 259-346

PTM

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
Proteolytically processed. The ectodomain is cleaved, probably by a metalloprotease, to produce a membrane-tethered C-terminal fragment. This fragment is then further processed by the gamma-secretase complex to yield a soluble intracellular domain peptide which can translocate to the nucleus. The intracellular domain peptide is highly labile suggesting that it is targeted for degradation by the proteasome.

Gürsoy, S., Hazan, F., Öztürk, T., Çolak, R., & Çalkavur, Ş. (2021). Evaluation of Sporadic and Familial Cases with Craniofrontonasal Syndrome: A Wide Clinical Spectrum and Identification of a Novel EFNB1 Gene Mutation. Molecular Syndromology, 12(5), 269-278.

Zhang, H., Cui, Z., Cheng, D., Du, Y., Guo, X., Gao, R., ... & Wang, C. (2021). RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis. Autophagy, 17(10), 3030-3047.

Shi, Y., Sun, Y., Cheng, H., & Wang, C. (2021). EFNB1 Acts as a Novel Prognosis Marker in Glioblastoma through Bioinformatics Methods and Experimental Validation. Journal of oncology, 2021.

Bukowska-Olech, E., Gawliński, P., Jakubiuk-Tomaszuk, A., Jędrzejowska, M., Obersztyn, E., Piechota, M., ... & Jamsheer, A. (2021). Clinical and molecular characterization of craniofrontonasal syndrome: new symptoms and novel pathogenic variants in the EFNB1 gene. Orphanet Journal of Rare Diseases, 16(1), 1-9.

Acosta‐Fernández, E., Zenteno, J. C., Chacón‐Camacho, O. F., Peña‐Padilla, C., Bobadilla‐Morales, L., Corona‐Rivera, A., ... & Corona‐Rivera, J. R. (2020). Extracranial midline defects in a patient with craniofrontonasal syndrome with a novel EFNB1 mutation. American Journal of Medical Genetics Part A, 182(5), 1223-1229.

Shotelersuk, V., Kamolvisit, W., Rojvachiranonda, N., Suphapeetiporn, K., Porntaveetus, T., & Shotelersuk, V. (2020). Severe craniofrontonasal syndrome in a male patient mosaic for a novel nonsense mutation in EFNB1. European Journal of Medical Genetics, 63(6), 103924.

Al Rahim, M., Yoon, Y., Dimovasili, C., Shao, Z., Huang, Q., Zhang, E., ... & Robakis, N. K. (2020). Presenilin1 familial Alzheimer disease mutants inactivate EFNB1-and BDNF-dependent neuroprotection against excitotoxicity by affecting neuroprotective complexes of N-methyl-d-aspartate receptor. Brain communications, 2(2), fcaa100.

Arthur, A., Nguyen, T. M., Paton, S., Zannettino, A. C., & Gronthos, S. (2019). Loss of EfnB1 in the osteogenic lineage compromises their capacity to support hematopoietic stem/progenitor cell maintenance. Experimental Hematology, 69, 43-53.

Jiang, Y., Qian, Y. Q., Yang, M. M., Zhan, Q. T., Chen, Y., Xi, F. F., ... & Luo, Q. (2019). Whole-exome sequencing revealed mutations of MED12 and EFNB1 in fetal agenesis of the corpus callosum. Frontiers in Genetics, 10, 1201.

Wu, T., Zhang, B. Q., Raelson, J., Yao, Y. M., Wu, H. D., Xu, Z. X., ... & Hu, S. J. (2018). Analysis of the association of EPHB6, EFNB1 and EFNB3 variants with hypertension risks in males with hypogonadism. Scientific reports, 8(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY3928)

Human Recombinant EFNB1 protein, His Tag (CAT#: V2LY-0526-LY3929)

Mouse Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY8271)

Mouse Recombinant EFNB1 protein, His Tag (CAT#: V2LY-0526-LY8272)

Rat Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY9366)

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Mouse Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY8271)

Mouse Anti-EFNB1 Recombinant Antibody (CBFYE-0505) (CAT#: CBMAB-E0859-FY)

Rat Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY9366)

Mouse Recombinant EFNB1 protein, His Tag (CAT#: V2LY-0526-LY8272)

Human Recombinant EFNB1 protein, His Tag (CAT#: V2LY-0526-LY3929)

Human Recombinant EFNB1 protein, hFc Tag (CAT#: V2LY-0526-LY3928)

Rabbit Anti-EFNB1 Recombinant Antibody (EG1041) (CAT#: V2LY-0425-LY1524)

Isotype control

For research use only. Not intended for any clinical use.

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