CDK5RAP3

CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include protein kinase binding and cyclin binding.

Full Name

CDK5 Regulatory Subunit Associated Protein 3

Function

Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress (PubMed:23152784, PubMed:30635284).
Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063).
Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517).
Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857).
Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922).
May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299).
May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).
Required for liver development (By similarity).
(Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.

Biological Process

Apoptotic nuclear changes Source: UniProtKB
Brain development Source: UniProtKB
Cell population proliferation Source: UniProtKB
Definitive erythrocyte differentiation Source: UniProtKB
Endoplasmic reticulum unfolded protein response Source: UniProtKB
Liver development Source: UniProtKB
Mitotic G2/M transition checkpoint Source: UniProtKB
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Negative regulation of cellular protein catabolic process Source: UniProtKB
Negative regulation of MAP kinase activity Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of protein kinase activity by regulation of protein phosphorylation Source: UniProtKB
Negative regulation of protein phosphorylation Source: UniProtKB
Negative regulation of protein serine/threonine kinase activity Source: UniProtKB
Positive regulation of protein localization to nucleus Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Positive regulation of signal transduction by p53 class mediator Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Protein ufmylation Source: MGI
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Regulation of mitotic cell cycle Source: GO_Central
Regulation of neuron differentiation Source: UniProtKB
Regulation of phosphatase activity Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Cytoplasm; Centrosome. Colocalizes and associates with microtubules.

PTM

May be phosphorylated by CDK5.
Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1.
May be ufmylated.
Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.