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Mouse Anti-CDK5RAP3 Recombinant Antibody (CBFYC-1612) (CBMAB-C1671-FY)

This product is mouse antibody that recognizes CDK5RAP3. The antibody CBFYC-1612 can be used for immunoassay techniques such as: ELISA, IF, IHC-P, WB.
See all CDK5RAP3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1612
Antibody Isotype
IgG1, k
Application
ELISA, IF, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CDK5 Regulatory Subunit Associated Protein 3
Introduction
CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include protein kinase binding and cyclin binding.
Entrez Gene ID
80279
UniProt ID
Q96JB5
Alternative Names
CDK5 Regulatory Subunit Associated Protein 3; LXXLL/Leucine-Zipper-Containing ARF-Binding Protein; LXXLL/Leucine-Zipper-Containing ARFbinding Protein; Ischemic Heart CDK5 Activator-Binding Protein C53; IC53; LZAP; CDK5 Regulatory Subunit Associated Protein IC53-2; CDK5 Regulatory Subunit-Associated Protein 3
Function
Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress (PubMed:23152784, PubMed:30635284).
Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063).
Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517).
Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857).
Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922).
May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299).
May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).
Required for liver development (By similarity).
(Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.
Biological Process
Apoptotic nuclear changes Source: UniProtKB
Brain development Source: UniProtKB
Cell population proliferation Source: UniProtKB
Definitive erythrocyte differentiation Source: UniProtKB
Endoplasmic reticulum unfolded protein response Source: UniProtKB
Liver development Source: UniProtKB
Mitotic G2/M transition checkpoint Source: UniProtKB
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Negative regulation of cellular protein catabolic process Source: UniProtKB
Negative regulation of MAP kinase activity Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of protein kinase activity by regulation of protein phosphorylation Source: UniProtKB
Negative regulation of protein phosphorylation Source: UniProtKB
Negative regulation of protein serine/threonine kinase activity Source: UniProtKB
Positive regulation of protein localization to nucleus Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Positive regulation of signal transduction by p53 class mediator Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Protein ufmylation Source: MGI
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Regulation of mitotic cell cycle Source: GO_Central
Regulation of neuron differentiation Source: UniProtKB
Regulation of phosphatase activity Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm; Centrosome. Colocalizes and associates with microtubules.
PTM
May be phosphorylated by CDK5.
Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1.
May be ufmylated.
Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.

Sheng, L., Li, J., Rao, S., Yang, Z., & Huang, Y. (2021). Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 3: Potential Functions and Implications for Development and Disease. Frontiers in Oncology, 4207.

Lin, J. X., Yoon, C., Li, P., Ryeom, S. W., Cho, S. J., Zheng, C. H., ... & Huang, C. M. (2020). CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer. British Journal of Cancer, 123(7), 1131-1144.

Yang, S., Yang, R., Wang, H., Huang, Y., & Jia, Y. (2020). CDK5RAP3 Deficiency Restrains Liver Regeneration after Partial Hepatectomy Triggering Endoplasmic Reticulum Stress. The American Journal of Pathology, 190(12), 2403-2416.

Egusquiaguirre, S. P., Liu, S., Tošić, I., Jiang, K., Walker, S. R., Nicolais, M., ... & Frank, D. A. (2020). CDK5RAP3 is a co-factor for the oncogenic transcription factor STAT3. Neoplasia, 22(1), 47-59.

Wang, J. B., Gao, Y. X., Lian, N. Z., Ma, Y. B., Li, P., Lin, J. X., ... & Huang, C. M. (2020). CDK5RAP3 as a Novel Biomarker Signature Predicting Survival and Adjuvant Chemotherapeutic Benefit in Gastric Cancer.

Li, J., Hu, X., Su, M., Shen, H., Qiu, W., & Tian, Y. (2019). CDK5RAP3 participates in autophagy regulation and is downregulated in renal cancer. Disease markers, 2019.

Chen, Q. Y., Liu, L. C., Wang, J. B., Xie, J. W., Lin, J. X., Lu, J., ... & Zheng, C. H. (2019). CDK5RAP3 inhibits the translocation of MCM6 to influence the prognosis in gastric cancer. Journal of Cancer, 10(19), 4488.

Lin, J. X., Weng, X. F., Xie, X. S., Lian, N. Z., Qiu, S. L., Wang, J. B., ... & Xie, J. W. (2019). CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling. Cancer cell international, 19(1), 1-16.

Zheng, C. H., Wang, J. B., Lin, M. Q., Zhang, P. Y., Liu, L. C., Lin, J. X., ... & Huang, C. M. (2018). CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. Journal of Experimental & Clinical Cancer Research, 37(1), 1-9.

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For research use only. Not intended for any clinical use.

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