Mouse Anti-AP4E1 Recombinant Antibody (32) (CBMAB-A2996-YC)

Name: Mouse Anti-AP4E1 Recombinant Antibody (32)
SKU: CBMAB-A2996-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AP4E1 Recombinant Antibody (32) in IP

RagA/B KO HEK293A cells were transfected with HA-tagged ArfGAP1, HA-tagged ArfGAP2, or RFP1 (control) for 24 h. Cell lysates were immunoprecipitated with anti-HA beads. Immunoprecipitates (IP) or whole cell lysate (WCL) samples were probed for HA and components of AP 1-5 complexes, and clathrin (CHC). ...View More

Meng, D., Yang, Q., Melick, C. H., Park, B. C., Hsieh, T. S., Curukovic, A., ... & Jewell, J. L. (2021). ArfGAP1 inhibits mTORC1 lysosomal localization and activation. The EMBO journal, 40(12), e106412.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

Application

WB, IP

Immunogen

Amino acids 685-793 of AP-4ε of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adaptor Related Protein Complex 4 Epsilon 1 Subunit

Introduction

AP4E1 is a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and

Entrez Gene ID

23431

UniProt ID

Q9UPM8

Alternative Names

Adaptor Related Protein Complex 4 Epsilon 1 Subunit; Adaptor-Related Protein Complex 4 Subunit Epsilon-1; AP-4 Adaptor Complex Subunit Epsilon; Epsilon-Adaptin; Adaptor-Related Protein Complex 4, Epsilon 1 Subunit; Adaptor-Related Protein Complex AP-4 Eps

Function

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed:10066790, PubMed:10436028). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (Probable).

Biological Process

Post-Golgi vesicle-mediated transport Source: Reactome
Protein localization Source: UniProtKB
Protein targeting Source: UniProtKB
Receptor-mediated endocytosis Source: GO_Central

Cellular Location

Trans-Golgi network membrane

Involvement in disease

Spastic paraplegia 51, autosomal recessive (SPG51): A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. SPG51 is a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest motor and posture impairments often associated with epilepsy and disturbances of cognition, behavior, sensation, and communication.
Stuttering, familial persistent 1 (STUT1): A familial form of stuttering, a disturbance in the normal fluency and time patterning of speech, characterized by frequent repetitions or prolongations of sounds or syllables, and by interruptions of speech known as blocks. STUT1 inheritance is autosomal dominant.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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