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Mouse Anti-AP4E1 Recombinant Antibody (32) (CBMAB-A2996-YC)

Provided herein is a Mouse monoclonal antibody against Human Adaptor Related Protein Complex 4 Epsilon 1 Subunit. The antibody can be used for immunoassay techniques, such as WB.
See all AP4E1 antibodies
Published Data
Mouse Anti-AP4E1 Recombinant Antibody (32) in IP
RagA/B KO HEK293A cells were transfected with HA-tagged ArfGAP1, HA-tagged ArfGAP2, or RFP1 (control) for 24 h. Cell lysates were immunoprecipitated with anti-HA beads. Immunoprecipitates (IP) or whole cell lysate (WCL) samples were probed for HA and components of AP 1-5 complexes, and clathrin (CHC). ...View More
Meng, D., Yang, Q., Melick, C. H., Park, B. C., Hsieh, T. S., Curukovic, A., ... & Jewell, J. L. (2021). ArfGAP1 inhibits mTORC1 lysosomal localization and activation. The EMBO journal, 40(12), e106412.

Summary

Host Animal
Mouse
Specificity
Human
Clone
32
Antibody Isotype
IgG1, κ
Application
WB, IP

Basic Information

Immunogen
Amino acids 685-793 of AP-4ε of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adaptor Related Protein Complex 4 Epsilon 1 Subunit
Introduction
AP4E1 is a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and
Entrez Gene ID
23431
UniProt ID
Q9UPM8
Alternative Names
Adaptor Related Protein Complex 4 Epsilon 1 Subunit; Adaptor-Related Protein Complex 4 Subunit Epsilon-1; AP-4 Adaptor Complex Subunit Epsilon; Epsilon-Adaptin; Adaptor-Related Protein Complex 4, Epsilon 1 Subunit; Adaptor-Related Protein Complex AP-4 Eps
Function
Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed:10066790, PubMed:10436028). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (Probable).
Biological Process
Post-Golgi vesicle-mediated transport Source: Reactome
Protein localization Source: UniProtKB
Protein targeting Source: UniProtKB
Receptor-mediated endocytosis Source: GO_Central
Cellular Location
Trans-Golgi network membrane
Involvement in disease
Spastic paraplegia 51, autosomal recessive (SPG51): A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. SPG51 is a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest motor and posture impairments often associated with epilepsy and disturbances of cognition, behavior, sensation, and communication.
Stuttering, familial persistent 1 (STUT1): A familial form of stuttering, a disturbance in the normal fluency and time patterning of speech, characterized by frequent repetitions or prolongations of sounds or syllables, and by interruptions of speech known as blocks. STUT1 inheritance is autosomal dominant.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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