Mouse Anti-AK4 Recombinant Antibody (V2-180419) (CBMAB-A1891-YC)

Name: Mouse Anti-AK4 Recombinant Antibody (V2-180419)
SKU: CBMAB-A1891-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AK4 Recombinant Antibody (CBYC-A472) in WB

Upregulated protein expression level of AK4 in PAAD tissues compared with the paired adjacent normal tissue specimens were revealed by western blotting. ...View More

Li, L., Deng, T., Zhang, Q., Yang, Y., Liu, Y., Yuan, L., & Xie, M. (2022). AK4P1 is a cancer‑promoting pseudogene in pancreatic adenocarcinoma cells whose transcripts can be transmitted by exosomes. Oncology Letters, 23(5), 1-9.

Mouse Anti-AK4 Recombinant Antibody (CBYC-A472) in WB and IF

Olfactory sensory neurons express AK in the cilia. (A-E) Immunocytochemistry of dissociated OSNs; (A) phase contrast; (B) anti-AK4 immunofluorescence; (C) overlay of A and B, with inset; (D) phase contrast of the control OSN; (E) immunofluorescence without primary anti-AK4 antibody. Bar: 5 μm. (F-H) Immunohistochemistry of an OE with anti-AK4 (F), anti-ACIII (G), and overlay of F and G (H). (I-K) Immunohistochemistry of a deciliated epithelium with anti-AK4 (I), anti-ACIII (J), and overlay of I and J (K). Bar 10 μm. (L) Immunoblot of an enriched ciliary membrane preparation, testing for the presence of AK4; quantification shown below (N = 4). (M) RT-PCR of dissociated OSNs; AK1 (left) and AK4 (right) cDNA (247 and 105 bp, respectively); positive and negative controls for AK1 are muscle and liver, and cerebellum and lung for AK4. N = 8. ...View More

Acevedo, C., Blanchard, K., Bacigalupo, J., & Vergara, C. (2019). Possible ATP trafficking by ATP-shuttles in the olfactory cilia and glucose transfer across the olfactory mucosa. FEBS letters, 593(6), 601-610.

Mouse Anti-AK4 Recombinant Antibody (CBYC-A472) in WB

Western blot analysis revealed the increased expression of AK4 protein in TamR MCF-7 cells. ...View More

Liu, X., Gonzalez, G., Dai, X., Miao, W., Yuan, J., Huang, M., ... & Wang, Y. (2020). Adenylate kinase 4 modulates the resistance of breast cancer cells to tamoxifen through an m6A-based epitranscriptomic mechanism. Molecular Therapy, 28(12), 2593-2604.

Mouse Anti-AK4 Recombinant Antibody (CBYC-A472) in IF

Validation of candidates by immunofluorescence staining in cerebellum. Candidates show prominent colocalization with cell-type-specific GFP-OMM (green), confirming the proteomics data GC. ...View More

Fecher, C., Trovò, L., Müller, S. A., Snaidero, N., Wettmarshausen, J., Heink, S., ... & Misgeld, T. (2019). Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. Nature neuroscience, 22(10), 1731-1742.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180419

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

N-terminus of AK4 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenylate Kinase 4

Introduction

AK4 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phos

Entrez Gene ID

205

UniProt ID

P27144

Alternative Names

Ak3; AK3; Ak-4; AK3L2; Ak-3; AK3L1; D4Ertd274e; AK 4; Ak3l1

Function

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor (PubMed:19073142, PubMed:19766732, PubMed:23416111). Also displays broad nucleoside diphosphate kinase activity (PubMed:19073142, PubMed:19766732, PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435).

Biological Process

ADP biosynthetic process Source: UniProtKB-UniRule
AMP metabolic process Source: BHF-UCL
ATP metabolic process Source: BHF-UCL
Brain development Source: Ensembl
Cellular response to hypoxia Source: UniProtKB
GTP metabolic process Source: BHF-UCL
Liver development Source: Ensembl
Nucleobase-containing small molecule interconversion Source: Reactome
Nucleoside diphosphate phosphorylation Source: UniProtKB
Nucleoside triphosphate biosynthetic process Source: UniProtKB
Regulation of ATP biosynthetic process Source: UniProtKB
Regulation of oxidative phosphorylation Source: UniProtKB
Response to drug Source: Ensembl

Cellular Location

Mitochondrion matrix

Chin, W. Y., He, C. Y., Chow, T. W., Yu, Q. Y., Lai, L. C., & Miaw, S. C. (2021). Adenylate Kinase 4 Promotes Inflammatory Gene Expression via Hif1α and AMPK in Macrophages. Frontiers in Immunology, 12.

Liu, X., Gonzalez, G., Dai, X., Miao, W., Yuan, J., Huang, M., ... & Wang, Y. (2020). Adenylate Kinase 4 Modulates the Resistance of Breast Cancer Cells to Tamoxifen through an m6A-Based Epitranscriptomic Mechanism. Molecular Therapy, 28(12), 2593-2604.

Jan, Y. H., Lai, T. C., Yang, C. J., Lin, Y. F., Huang, M. S., & Hsiao, M. (2019). Adenylate kinase 4 modulates oxidative stress and stabilizes HIF-1α to drive lung adenocarcinoma metastasis. Journal of hematology & oncology, 12(1), 1-14.

Xin, F., Yao, D. W., Fan, L., Liu, J. H., & Liu, X. D. (2019). Adenylate kinase 4 promotes bladder cancer cell proliferation and invasion. Clinical and experimental medicine, 19(4), 525-534.

Jan, Y. H., Lai, T. C., Yang, C. J., Huang, M. S., & Hsiao, M. (2019). A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma. Scientific reports, 9(1), 1-11.

Wujak, M., Wilke, T., Weiss, A., Brosien, M., Wu, C. Y., Schermuly, R. T., ... & Weissmann, N. (2019). Role of adenylate kinase 4 as a new metabolic regulator of pulmonary artery smooth muscle cells under hypoxia.

Wujak, M., Brosien, M., Wu, C. Y., Schermuly, R. T., Ghofrani, H. A., Seeger, W., ... & Weissmann, N. (2019). Emerging Role of Adenylate Kinase 4 as a Metabolic Player Regulating Cellular Hypoxic Responses in Pulmonary Cells. In C60. VASCULAR BIOLOGY IN LUNG DISEASE (pp. A5287-A5287). American Thoracic Society.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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