NCBP2

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

nuclear cap binding protein subunit 2, 20kDa

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).

Alternative mRNA splicing, via spliceosome Source: ComplexPortal
Cap-dependent translational initiation Source: ComplexPortal
Gene silencing by miRNA Source: ComplexPortal
Histone mRNA metabolic process Source: ComplexPortal
mRNA 3'-end processing Source: ComplexPortal
mRNA cis splicing, via spliceosome Source: UniProtKB
mRNA export from nucleus Source: ComplexPortal
mRNA splicing, via spliceosome Source: GO_Central
mRNA transcription by RNA polymerase II Source: ComplexPortal
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Positive regulation of mRNA 3'-end processing Source: UniProtKB
Positive regulation of phosphorylation of RNA polymerase II C-terminal domain Source: ComplexPortal
Positive regulation of RNA export from nucleus Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Pre-mRNA cleavage required for polyadenylation Source: UniProtKB
Primary miRNA processing Source: ComplexPortal
Regulation of translational initiation Source: UniProtKB
RNA interference Source: ComplexPortal
RNA splicing Source: UniProtKB
snRNA export from nucleus Source: UniProtKB

Nucleus
Cytoplasm